Transgenic GNA expressing potato plants augment the beneficial biocontrol of Lacanobia oleracea (Lepidoptera; Noctuidae) by the parasitoid Eulophus pennicornis (Hymenoptera; eulophidae)
Transgenic GNA expressing potato plants augment the beneficial biocontrol of Lacanobia oleracea (Lepidoptera; Noctuidae) by the parasitoid Eulophus pennicornis (Hymenoptera; eulophidae)
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DOI：
10.1023/a:1008923103515
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发表时间：
2001-02-01
影响因子：
3
通讯作者：
Gatehouse, AMR
中科院分区：
文献类型：
--
作者：
Bell, HA;Fitches, EC;Gatehouse, AMR
在温室试验中，研究了在转基因马铃薯中表达雪花莲凝集素（Galanthus nivalis凝集素，GNA）基因对群居性外寄生蜂小圆蛾（Eulophus pennicornis）寄生植物性害虫Lacanobia oleracea的影响。GNA的表达(约。1.0%总可溶性蛋白)的转基因植株显著降低了害虫危害水平，从而证实了之前的研究。此外，与没有寄生蜂的植株相比，有寄生蜂的转基因植株和对照植株受到的伤害水平显著降低。对于表达GNA的植物，寄生蜂的存在导致害虫物种造成的损害水平进一步降低（约21%）。寄生蜂寄生并随后在害虫幼虫上发育的能力并未因寄主饮食中GNA的存在而改变。在表达GNA的马铃薯植株上发育的小蜂后代与在对照马铃薯植株上发育的小蜂后代相比，繁殖力没有显著变化，但雌性寄生蜂的平均体型和寿命显著降低。寄主取食表达gna的植株产生的F-2子代数量与寄主取食对照植株产生的F-2子代数量无显著差异。本研究结果表明，使用表达杀虫蛋白的转基因植物与部署有益昆虫是相容的，两者可能以积极的方式相互作用。
The effect of expressing the gene encoding snowdrop lectin (Galanthus nivalis agglutinin, GNA) in transgenic potato plants, on parasitism of the phytophagous insect pest Lacanobia oleracea by the gregarious ectoparasitoid Eulophus pennicornis, was investigated in glasshouse trials. Expression of GNA (approx. 1.0% total soluble protein) by transgenic plants significantly reduced the level of pest damage, thus confirming previous studies. Furthermore, the presence of the parasitoid significantly reduced the levels of damage incurred either by the transgenic or control plants when compared to those plants grown in the absence of the parasitoid. For the GNA expressing plants the presence of the parasitoid resulted in further reductions (ca. 21%) in the level of damage caused by the pest species. The ability of the wasp to parasitise and subsequently develop on the pest larvae was not altered by the presence of GNA in the diet of the host. E. pennicornis progeny that developed on L. oleracea reared on GNA expressing plants showed no significant alteration in fecundity when compared with wasps that had developed on hosts fed on control potato plants, although mean size and longevity of female parasitoids was significantly reduced. The number of F-2 progeny produced by parasitoids derived from hosts fed on GNA-expressing plants was not significantly different to those produced by parasitoids from hosts fed control plants. Results from the present study demonstrate that the use of transgenic plants expressing insecticidal proteins can be compatible with the deployment of beneficial insects and that the two factors may interact in a positive manner.