Stimulation of renal deoxyribonucleic acid synthesis by a pituitary-derived renotropin and its inhibition by testosterone and thyroxine.
Stimulation of renal deoxyribonucleic acid synthesis by a pituitary-derived renotropin and its inhibition by testosterone and thyroxine.
复制标题
垂体衍生的促肾上腺素刺激肾脏脱氧核糖核酸合成，并受睾酮和甲状腺素抑制。
作者：
K. Nomura;H. Demura;K. Shizume
通过注射几种不同的促性腺激素制剂（两种羊LH部分，一种牛LH部分和一种hCG部分）或对照物，获得去势垂体切除的雄性大鼠肾脏切片，然后在含有[3H]胸腺嘧啶的缓冲液中孵育。上述只有一种羊LH制剂增加了[3H]胸腺嘧啶与肾脏DNA的结合，在注射后8-10小时出现峰值，因此称为促肾上腺素。然而，在睾丸完整的大鼠中，没有观察到这种效果。此外，虽然单独丙酸睾酮不会改变去势垂体切除大鼠的基础掺入，但它可以消除促肾上腺素刺激的掺入。这些结果表明，雄激素，无论是来自睾丸还是外源性给药，都能抑制促肾上腺素刺激的[3H]胸苷的增加。雄激素对T4也有这种拮抗作用，但程度较轻。我们的研究结果证实了肾促肾上腺素的存在，这不能归因于其他已知的垂体激素，并表明该因子与另外两种促进肾脏生长的激素睾酮和T4之间存在复杂的相互作用。
Kidney slices were obtained from castrated-hypophysectomized male rats treated with a single injection of several different gonadotropin preparations (two ovine LH fractions, one bovine LH fraction, and one hCG fraction) or vehicle, then incubated in a buffer containing [3H]thymidine. Only one of the above, an ovine LH preparation, increased [3H]thymidine incorporation into renal DNA, with a peak occurring 8-10 h after injection and therefore termed renotropin. However, in hypophysectomized rats with intact testes, such an effect was not observed. Furthermore, while testosterone propionate alone did not alter basal incorporation in castrated-hypophysectomized rats, it abolished the incorporation that was stimulated by renotropin. These results suggest that androgens, whether of testicular origin or exogenously administered, suppress the increased incorporation of [3H]thymidine stimulated by renotropin. This antagonistic effect of androgen was also observed with T4, but to a lesser degree. Our findings confirm the presence of renotropin, which could not be attributed to other known pituitary hormones, and suggest that there is a complex interaction between this factor and two other renal growth-promoting hormones, testosterone and T4.
小鼠肾脏鸟氨酸脱羧酶活性的雄激素调节及其与细胞质和核雄激素受体浓度变化的关系。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Pajunen,AE;Isomaa,VV;Jänne,OA;Bardin,CW
通讯作者：
Bardin,CW
绵羊垂体促肾素部分的部分纯化和表征。
DOI：
10.1073/pnas.79.21.6675
发表时间：
1982
影响因子：
11.1
作者：
Nomura,K;Puett,D;Nicholson,WE;Liddle,GW
通讯作者：
Liddle,GW