Analysis of the involvement of the von Willebrand factor-glycoprotein Ib interaction in platelet adhesion to a collagen-coated surface under flow conditions.
Analysis of the involvement of the von Willebrand factor-glycoprotein Ib interaction in platelet adhesion to a collagen-coated surface under flow conditions.
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分析流动条件下血小板粘附到胶原涂层表面时冯维勒布兰德因子-糖蛋白 Ib 相互作用的参与情况。
作者：
M. Moroi;Stephanie M Jung;S. Nomura;S. Sekiguchi;A. Ordinas;M. Díaz
在体内流动的血液中形成血栓的必要初始反应是血小板与细胞外基质暴露表面的粘连。血浆中的von Willebrand因子(VWF)和血小板膜上的糖蛋白(GP)Ib在血小板黏附中的作用已经得到了充分的研究。我们最近开发了一种在流动条件下测量血小板粘附性的方法(“流动粘附法”)，使我们能够表征血小板与胶原蛋白涂层表面的粘附性。在这里，我们应用我们的方法来分析血小板与vWF涂层表面的粘附性，以确定这与胶原涂层表面的粘附性有何不同。血小板与vWF涂层表面的黏附被监测为黏附的血小板所占据的面积呈线性增加。荧光图像显示，黏附在vWF表面的血小板主要是单个血小板，如果有的话，血小板聚集物很小，这是与胶原表面黏附的主要区别，而胶原表面黏附的血小板大多是聚集态的。流式粘附法检测到vWF表面的血小板运动，提示vWF与血小板的可逆结合。在较高的切变率或较低的表面vWF密度下，血小板的速度增加。在血流开始前用聚集剂Botrocetin处理vWF涂层表面，可增加血小板粘附性，同时显著降低血小板运动速度。目前对经vWF处理的胶原表面血小板粘附性的观察和对胶原表面血小板运动速度的测量表明，vWF分子与胶原表面的第一次相互作用是vWF分子与胶原表面的结合。这种少量的vWF分子将起到吸引和减缓靠近表面的血小板流动的作用。这将促进主要由包括GP Ia/IIa和GP VI在内的血小板胶原受体与胶原相互作用而产生的对胶原表面的实际黏附。
The requisite initial reaction for in vivo thrombus formation in flowing blood is platelet adhesion to the exposed surface of the extracellular matrix. The contribution of von Willebrand factor (vWF ) in plasma and glycoprotein (GP) Ib on the platelet membrane to platelet adhesion has been well-documented. We have recently developed a procedure (the "flow adhesion assay") for measuring platelet adhesion under flow conditions that allowed us to characterize platelet adhesion to a collagen-coated surface. Here, we apply our method to analyze platelet adhesion to a vWF-coated surface to determine how this might differ from adhesion to a collagen-coated surface. Platelet adhesion to the vWF-coated surface was monitored as the linear increase in the area occupied by adherent platelets. The fluorescence image showed that platelets adhering to the vWF surface were mainly single platelets, and if any were present, the platelet aggregates were small, this being the primary difference from the adhesion to a collagen surface, where adherent platelets were mostly in aggregates. The flow adhesion assay detected the movement of platelets on the vWF surface, suggesting the reversible binding of vWF with platelets. The velocity of the platelets increased at higher shear rates or at lower vWF densities on the surface. Treatment of the vWF-coated surface with the aggregating agent botrocetin before initiation of blood flow increased platelet adhesion while dramatically decreasing the velocity of platelet movement. The present observations on the adhesion of platelets to the vWF-pretreated collagen surface and measurements of the velocity of platelets moving on the collagen surface suggest that the first interaction on the collagen-coated surface is the binding of vWF molecules to the collagen surface. This small number of vWF molecules would serve to attract and slow platelets flowing near the surface. This would facilitate the actual adhesion to the collagen surface that is mainly generated by the interaction between platelet collagen receptors, including GP Ia/IIa and GP VI, with collagen.
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DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Savage,B;Shattil,SJ;Ruggeri,ZM
通讯作者：
Ruggeri,ZM
缺乏功能性 CD36（糖蛋白 IV）的血小板在流动的全血中显示出与胶原蛋白的粘附力降低。
影响因子：
20.3
作者：
Diaz-Ricart,M;Tandon,NN;Carretero,M;Ordinas,A;Bastida,E;Jamieson,GA
通讯作者：
Jamieson,GA
鉴定参与与 botrocetin 形成复合物的不连续冯维勒布兰德因子序列。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Sugimoto,M;Mohri,H;McClintock,RA;Ruggeri,ZM
通讯作者：
Ruggeri,ZM
影响因子：
15.9
作者：
IKEDA, Y;HANDA, M;RUGGERI, ZM
通讯作者：
RUGGERI, ZM
人血管性血友病因子中单体 III 型胶原蛋白结合位点的定位。
影响因子：
2.9
作者：
Roth,GJ;Titani,K;Hoyer,LW;Hickey,MJ
通讯作者：
Hickey,MJ