Synthetic maize centromeres transmit chromosomes across generations
Synthetic maize centromeres transmit chromosomes across generations
复制标题
DOI：
10.1038/s41477-023-01370-8
复制
发表时间：
2022-09
期刊：
影响因子：
18
通讯作者：
R. Dawe;J. Gent;Yibing Zeng;Han Zhang;Fang-fang Fu;Kyle W. Swentowsky;D. W. Kim;Na Wang;Jianing Liu;Rebecca D. Piri
中科院分区：
文献类型：
--
作者：
R. Dawe;J. Gent;Yibing Zeng;Han Zhang;Fang-fang Fu;Kyle W. Swentowsky;D. W. Kim;Na Wang;Jianing Liu;Rebecca D. Piri
着丝粒是基因组的长的、经常重复的区域，其结合动粒蛋白并确保正常的染色体分离。工程着丝粒在体内的功能已被证明是困难的。在这里，我们描述了一个拴系的方法，激活功能玉米着丝粒在合成序列阵列。LexA-CENH 3融合蛋白用于将天然着丝粒组蛋白H3（CENH 3）招募到染色体臂上的LexO重复序列长阵列中。新招募的CENH 3足以组织功能性动粒，导致染色体断裂，释放染色体片段，通过减数分裂并进入后代。几个片段形成独立的新染色体与着丝粒定位在LexO重复阵列。新的着丝粒是自我维持的，并在没有LexA-CENH 3激活剂的情况下将新染色体传递给后代。我们的研究结果表明，使用人工合成的着丝粒核型工程应用的可行性。
Centromeres are long, often repetitive regions of genomes that bind kinetochore proteins and ensure normal chromosome segregation. Engineering centromeres that function in vivo has proven to be difficult. Here we describe a tethering approach that activates functional maize centromeres at synthetic sequence arrays. A LexA-CENH3 fusion protein was used to recruit native Centromeric Histone H3 (CENH3) to long arrays of LexO repeats on a chromosome arm. Newly recruited CENH3 was sufficient to organize functional kinetochores that caused chromosome breakage, releasing chromosome fragments that were passed through meiosis and into progeny. Several fragments formed independent neochromosomes with centromeres localized over the LexO repeat arrays. The new centromeres were self-sustaining and transmitted neochromosomes to subsequent generations in the absence of the LexA-CENH3 activator. Our results demonstrate the feasibility of using synthetic centromeres for karyotype engineering applications.