Effect of potassium perfluorooctanesulfonate, perfluorooctanoate and octanesulfonate on the phase transition of dipalmitoylphosphatidylcholine (DPPC) bilayers.
Effect of potassium perfluorooctanesulfonate, perfluorooctanoate and octanesulfonate on the phase transition of dipalmitoylphosphatidylcholine (DPPC) bilayers.
复制标题
全氟辛烷磺酸钾、全氟辛酸和辛烷磺酸对二棕榈酰磷脂酰胆碱 (DPPC) 双层相变的影响。
DOI：
10.1016/j.bbamem.2007.02.003
复制
发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Lehmler,H-J
中科院分区：
文献类型：
--
作者：
Xie,W;Kania-Korwel,I;Bummer,PM;Lehmler,H-J
全氟辛烷磺酸（PFOS）是一种持久性环境污染物，可能通过抑制肺表面活性物质造成不良影响。为了进一步了解这种潜在的毒性机制，我们使用稳态荧光各向异性光谱和 DSC（差示扫描量热法）研究了 PFOS 钾盐与二棕榈酰磷脂酰胆碱 (DPPC)（肺表面活性剂的主要成分）的相互作用。此外，我们还研究了两种结构相关的化合物全氟辛酸 (PFOA) 和辛磺酸 (OS) 钾盐与 DPPC 的相互作用。在荧光实验中，使用 1,6-二苯基-1,3,5-己三烯 (DPH) 和 1-(4-三甲基铵苯基)-6-苯基-1,3,5-己三烯对甲苯磺酸盐 (TMA-DPH) 作为荧光探针，观察到随着所有三种化合物浓度的增加，DPPC (Tm) 的主相变温度线性降低，峰宽度增加。 PFOS 在低得多的浓度下会对 Tmand 峰宽产生影响，因为它在 DPPC 双层上的分配趋势增加，即 K(PFOS)>K(PFOA)>>K(OS) 中的分配系数降低。与荧光各向异性测量类似，所有三种化合物均在 DSC 实验中引起主相变温度开始时的线性降低和显着的峰展宽，其中 PFOS 对峰宽的影响最明显。 PFOS 和其他氟化表面活性剂对单层和双层 DPPC 的影响可能是这些化合物引起不良生物效应的机制之一。
Perfluorooctanesulfonic acid (PFOS) is a persistent environmental pollutant that may cause adverse effects by inhibiting pulmonary surfactant. To gain further insights in this potential mechanism of toxicity, we investigated the interaction of PFOS potassium salt with dipalmitoylphosphatidylcholine (DPPC) – the major component of pulmonary surfactant – using steady-state fluorescence anisotropy spectroscopy and DSC (differential scanning calorimetry). In addition, we investigated the interactions of two structurally related compounds, perfluorooctanoic acid (PFOA) and octanesulfonic acid (OS) potassium salt, with DPPC. In the fluorescence experiments a linear depression of the main phase transition temperature of DPPC (Tm) and an increased peak width was observed with increasing concentration of all three compounds, both using 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene p-toluenesulfonate (TMA-DPH) as fluorescent probes. PFOS caused an effect on Tmand peak width at much lower concentrations because of its increased tendency to partition onto DPPC bilayers, i.e., the partition coefficients decrease in the K(PFOS)>K(PFOA)>>K(OS). Similar to the fluorescence anisotropy measurements, all three compounds caused a linear depression in the onset of the main phase transition temperature and a significant peak broadening in the DSC experiments, with PFOS having the most pronounced effect of the peak width. The effect of PFOS and other fluorinated surfactants on DPPC in both mono- and bilayers may be one mechanism by which these compounds cause adverse biological effects.