The assimilation of sulfur from multiple sources and its correlation with expression of the sulfate-starvation-induced stimulon in Pseudomonas putida S-313
The assimilation of sulfur from multiple sources and its correlation with expression of the sulfate-starvation-induced stimulon in Pseudomonas putida S-313
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DOI：
10.1099/13500872-142-8-1989
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发表时间：
1996-08-01
期刊：
影响因子：
--
通讯作者：
Cook, AM
中科院分区：
文献类型：
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作者：
Beil, S;Kertesz, MA;Cook, AM
对恶臭假单胞菌S-313在限硫条件下的分批培养条件进行了优化。恶臭假单胞菌以硫酸盐为唯一硫源时呈指数生长，并伴随着硫酸盐的利用而生长直至耗尽。另外20%的蛋白质在硫酸盐明显消失后合成。计算细胞材料中所利用的硫酸盐的质量平衡，给出约3.6kg蛋白质（mol S）（-1）的观察到的摩尔生长产率和干物质中0.41%S的硫含量。半胱氨酸和硫氰酸盐的生长获得了类似的数据。该生物体也以4-甲苯磺酸盐（TS）作为硫源呈指数生长，基本上与硫酸盐相同，不同之处在于观察到TS耗尽后可忽略不计的蛋白质形成。用4-硝基儿茶酚硫酸盐（NCS）和乙磺酸盐也获得了类似的数据。同时使用选自硫酸盐、半胱氨酸和硫氰酸盐的任何底物对，尽管底物对中的一种总是优选的，但当仅保留一种底物时，生长以相同的速率继续。观察底物耗尽后的生长。从TS、NCS和乙磺酸盐中选择的任何底物对给出类似的数据，但在硫源耗尽后生长较少。如果从两组中选择混合底物对，则最初仅使用来自第一组的硫源，随后在滞后期后使用第二硫源。数据被认为反映了由硫酸盐饥饿诱导的刺激子施加的对硫的清除和硫在细胞中的分布的控制[Kertesz，Leisinger & Cook，J Bacteriol（1993）175，1187-1189]。
Conditions were optimized for the batch growth of Pseudomonas putida S-313 under sulfur-limited conditions. P. putida grew exponentially with sulfate as the sole source of sulfur, and growth was concomitant with the utilization of sulfate until it was exhausted. A further 20% of protein was synthesized after the apparent disappearance of sulfate. A mass balance for the utilized sulfate in cell material was calculated, given the observed molar growth yield of about 3.6 kg protein (mol S)(-1) and a sulfur content of 0.41% S in dry matter. Similar data were obtained for growth with cysteine and thiocyanate. The organism also grew exponentially with 4-toluenesulfonate (TS) as sulfur source, essentially as observed with sulfate, except that negligible protein formation after exhaustion of TS was observed. Similar data were also obtained with 4-nitrocatecholsulfate (NCS) and ethanesulfonate. Any substrate pair selected from sulfate, cysteine and thiocyanate was utilized simultaneously, and although one of the pair of substrates was always preferred, growth continued at the same rate when only one substrate remained. Growth after substrate exhaustion was observed. Any substrate pair selected from TS, NCS and ethanesulfonate gave similar data, but with less growth after exhaustion of the sulfur sources. If a mixed substrate pair was chosen from the two groups, the sulfur source from the first-named group was initially used exclusively, and the second source of sulfur was utilized subsequently, after a lag phase. The data are considered to reflect the control of scavenging for sulfur and of distribution of sulfur in the cell exerted by the sulfate-starvation-induced stimulon [Kertesz, Leisinger & Cook, J Bacteriol (1993) 175, 1187-1189].