Experimental models for analysis of oligodendrocyte pathophysiology in stroke.
Experimental models for analysis of oligodendrocyte pathophysiology in stroke.
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DOI：
10.1186/2040-7378-1-6
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发表时间：
2009-10-24
期刊：
影响因子：
--
通讯作者：
Lo EH
中科院分区：
文献类型：
--
作者：
Arai K;Lo EH
白色物质损害是中风的临床重要部分。然而，与灰质中神经元损伤的机制相比，白色物质的病理生理学仍然相对研究不足和了解不多。这篇简短的综述旨在总结目前对实验系统的认识，以分析白色物质损伤在卒中中的作用。体外平台包含成熟少突胶质细胞（OLG）以及少突胶质细胞前体细胞（OPC）的原代培养物。组织平台涉及视神经系统的制备。全动物平台包括脑缺血的体内模型，其试图靶向白色脑区域。虽然没有一个完美的模型系统，这些实验方法的收集最近允许更好地了解OLG/OPC损伤和脱髓鞘的分子和细胞途径。系统地利用这些细胞、组织和整个动物平台可能最终导致我们发现治疗中风和其他CNS疾病中的白色物质损伤的新靶点。
White matter damage is a clinically important part of stroke. However, compared to the mechanisms of neuronal injury in gray matter, white matter pathophysiology remains relatively understudied and poorly understood. This mini-review aims at summarizing current knowledge on experimental systems for analyzing the role of white matter injury relevant to stroke. In vitro platforms comprise primary cultures of both mature oligodendrocytes (OLGs) as well as oligodendrocyte precursor cells (OPCs). Tissue platforms involve preparations of optic nerve systems. Whole-animal platforms comprise in vivo models of cerebral ischemia that attempt to target white matter brain areas. While there is no single perfect model system, the collection of these experimental approaches have recently allowed a better understanding of the molecular and cellular pathways underlying OLG/OPC damage and demyelination. A systematic utilization of these cell, tissue and whole-animal platforms may eventually lead us to discover new targets for treating white matter injury in stroke and other CNS disorders.