A novel dual-channel Schiff base fluorescent chemo-sensor for Zn2 and Ca2 recognition: Synthesis, mechanism and application
A novel dual-channel Schiff base fluorescent chemo-sensor for Zn2 and Ca2 recognition: Synthesis, mechanism and application
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一种新型双通道席夫碱荧光化学传感器，用于Zn2和Ca2识别：合成、机制和应用
DOI：
10.1016/j.dyepig.2019.107614
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发表时间：
2019
影响因子：
4.5
通讯作者：
Xu Hongyao
中科院分区：
文献类型：
--
作者：
Zhao Gang;Guo Binyuan;Wei Gang;Guang Shanyi;Gu Zhengye;Xu Hongyao
成功设计并合成了一种将席夫碱、罗丹明B酰肼、水合肼基团融入三嗪中的新型多功能化学传感器（RHCS-NH2）。结果发现，RHCS-NH2传感器对CH3CN-H2O（v/v=9/1，Tris-HCl pH 7.0）中的Ca2+和DMF-H2O（95/5，v/v）中的Zn2+表现出立即多重响应。优化条件下，CH3CN-H2O（v/v=9/1，Tris-HCl pH 7.0）中Ca2+离子的线性范围为0.01~10.0μM，DMF-H2O（95/5，v/v）中Zn2+离子的线性范围为0.03~10.0μM，Ca2+和Zn2+离子的检出限为0.10nM。 Zn2+ 分别为 0.14nM。分别基于荧光滴定、乔布图和1H NMR滴定曲线、ESI-MS测量和理论计算，详细研究了RHCS-NH2与金属离子的反应机理。同时，3-(4,5)-二甲基硫杂氮(-z-y1)-3,5-二苯基四氮唑啉(-z-y1)-3,5-二苯四唑溴化物(MTT)实验和倒置荧光显微镜成像也证明该探针具有良好的细胞膜通透性和低毒性，表明其在体外具有潜在的适用性。
A novel multifunctional chemo-sensor (RHCS-NH2), incorporating Schiff base, rhodamine B hydrazide, hydrazine hydrate group into triazine, was successfully designed and synthesized. It was found that the RHCS-NH2sensor displays immediate multi-response toward Ca2+in CH3CN-H2O (v/v = 9/1, Tris-HCl pH 7.0) and Zn2+in DMF-H2O (95/5, v/v). Under the optimized conditions, the linear range of identifing Ca2+ions are from 0.01 to 10.0 μM in CH3CN-H2O (v/v = 9/1, Tris-HCl pH 7.0), 0.03–10.0 μM for Zn2+ions in DMF-H2O (95/5, v/v), and the detection limit were measured to be 0.10 nM for Ca2+and 0.14 nM for Zn2+, respectively. The reaction mechanism between RHCS-NH2and metal ions was investigated in detail based on fluorescence titration, Job's plots and1H NMR titration curve, ESI-MS measurement and theoretical calculation respectively. Simultaneously, 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assays and inverted fluorescence microscopy imaging also demonstrated that the probe has well cell membrane permeability and hypotoxicity, indicating its potential applicability in vitro.