A new approach using micro HPLC-MS/MS for multi-mycotoxin analysis in maize samples
A new approach using micro HPLC-MS/MS for multi-mycotoxin analysis in maize samples
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DOI：
10.1007/s12550-015-0221-y
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发表时间：
2015-05-01
影响因子：
3
通讯作者：
Humpf, Hans-Ulrich
中科院分区：
文献类型：
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作者：
Hickert, Sebastian;Gerding, Johannes;Humpf, Hans-Ulrich
采用微型高效液相色谱-串联质谱（HPLC-MS/MS）技术，建立了26种真菌毒素的定量分析方法。样品制备包括单一提取步骤和稀释-拍摄方法，无需进一步净化。总运行时间为9 min，每次色谱运行的溶剂消耗量低于0.3 mL，所提出的方法具有成本效益。欧盟委员会规定的谷物产品中最高或指导水平的所有毒素（伏马菌素B-1和B-2（FB 1和FB 2））;脱氧雪腐镰刀菌烯醇（DON）;黄曲霉毒素B-1、G（1）、B-2和G（2）（AFB（1）、AFG（1）、AFB（2）和AFG（2））;赭曲霉毒素A（OTA）; T-2和HT-2毒素;以及玉米赤霉烯酮（ZEN）。此外，还可以定量恩镰孢菌素B、B1、A和A1（EnB、EnB 1、EnA和EnA 1）;白僵菌素（B12）; 3-乙酰基-脱氧雪腐镰刀菌烯醇（3-AcDON）;镰刀菌素C（FusC）;杂色曲霉素（STC）;胶霉毒素（GT）;以及链格孢属毒素交链孢醇（AOH）、交链孢醇单甲醚（AME）、链格孢烯（ALT）、藤毒素（TEN）和链格孢毒素I（ATX I）.对于所有受管制的化合物，回收率在76%至120%之间。对于所有其他毒素，回收率至少为51%。该方法被应用于42个玉米样品在南非田间试验的分析。
Using micro high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) a simple and fast method for the quantitative determination of 26 mycotoxins was developed. Sample preparation consists of a single extraction step and a dilute-and-shoot approach without further cleanup. With a total run time of 9 min and solvent consumption below 0.3 mL per chromatographic run, the presented method is cost-effective. All toxins regulated by the European Commission with maximum or guidance levels in grain products (fumonisins B-1 and B-2 (FB1 and FB2)); deoxynivalenol (DON); aflatoxins B-1, G(1), B-2, and G(2) (AFB(1), AFG(1), AFB(2), and AFG(2)); ochratoxin A (OTA); T-2 and HT-2 toxins; and zearalenone (ZEN) can be quantified with this method. Furthermore, the enniatins B, B1, A, and A1 (EnB, EnB1, EnA, and EnA1); beauvericin (BEA); 3-acetyl- deoxynivalenol (3-AcDON); fusarin C (FusC); sterigmatocystin (STC); gliotoxin (GT); and the Alternaria toxins alternariol (AOH), alternariol monomethyl ether (AME), altenuene (ALT), tentoxin (TEN), and altertoxin I (ATX I) can also be quantified. For all regulated compounds, recoveries ranged between 76 and 120 %. For all other toxins, the recovery was at least 51 %. The method was applied for the analysis of 42 maize samples from field trials in South Africa.