RATE OF LOSS OF TRITIATED-THYMIDINE LABEL IN BASAL CELLS IN MOUSE EPITHELIAL TISSUES
RATE OF LOSS OF TRITIATED-THYMIDINE LABEL IN BASAL CELLS IN MOUSE EPITHELIAL TISSUES
复制标题
DOI：
10.1111/j.1365-2184.1986.tb00684.x
复制
发表时间：
1986-05-01
期刊：
影响因子：
--
通讯作者：
MACKENZIE, IC
中科院分区：
文献类型：
--
作者：
BICKENBACH, JR;MCCUTECHEON, J;MACKENZIE, IC
以前在小鼠和仓鼠的皮肤和口腔粘膜中发现了一种保留氚标记的上皮细胞亚群(称为标记保留细胞，LRC)。为了检测LRC数量减少的速度和标记程度的变化，用氚胸苷标记幼鼠，并评估标记从基底角质形成细胞稀释到90天的速度。标记后15天到90天，每个被检查的组织中LRC的数量减少，表皮组织保持的LRCs比例高于口腔粘膜。各时间段各组织中LRCs的颗粒计数表明，覆盖LRCs的银粒数也随着时间的推移而减少。观察到的LRC数量的减少及其标记程度随时间的变化表明，这些细胞分裂缓慢，这与干细胞有关。
A subpopulation of epithelial cells which retains a tritiated thymidine label (termed label-retaining cells, LRCs) has been previously demonstrated in skin and oral mucosae of mice and hamsters. To examine the rate of decrease in the number of LRCs and the changes in degree of labelling, young mice were labelled with tritiated thymidine and the rate at which label was diluted from basal keratinocytes assessed for up to 90 days. The number of LRCs in each tissue examined decreased from 15 to 90 days after labelling with the epidermal tissue maintaining a higher percentage of LRCs than the oral mucosae. Grain counts for LRCs in each tissue at each time period indicated that the number of silver grains overlying LRCs also decreased with time. The observed decrease in number of LRCs and the change in their degree of labelling with time suggest that such cells divide slowly, a property associated with stem cells.