Regioregular Alternating Polyampholytes Have Enhanced Biomimetic Ice Recrystallization Activity Compared to Random Copolymers and the Role of Side Chain versus Main Chain Hydrophobicity.
Regioregular Alternating Polyampholytes Have Enhanced Biomimetic Ice Recrystallization Activity Compared to Random Copolymers and the Role of Side Chain versus Main Chain Hydrophobicity.
复制标题
与随机共聚物以及侧链与主链疏水性的作用相比，区域性交替的多型聚糖具有增强的仿生冰层重结晶活性。
DOI：
10.1021/acs.biomac.6b01691
复制
发表时间：
2017-01-09
影响因子：
6.2
通讯作者：
Gibson MI
中科院分区：
文献类型：
--
作者：
Stubbs C;Lipecki J;Gibson MI
来自极地鱼类的抗冻蛋白是有效的冰重结晶抑制剂（IRI），可有效阻止所有冰的生长。具有IRI活性的添加剂已显示出增强细胞冷冻保存，具有改善供体细胞和组织分布的潜力。两性聚电解质，具有阴离子和阳离子侧链的聚合物，是一类迅速出现的聚合物冷冻保护剂，但其作用模式和活性所必需的结构特征尚不清楚。在这里，由马来酸酐共聚物合成区域规则性聚两性电解质，以实现带电基团的化学计量安装，确保区域规则性，这是使用常规无规共聚不可能的。采用模块化合成策略以使主链和侧链的疏水性能够变化，发现侧链的疏水性对IRI活性具有深远的影响。区域规整聚合物的活性被认为是上级那些来自一个标准的无规共聚与统计纳入的单体，表明序列组成是至关重要的IRI活性两性聚电解质的活性。
Antifreeze proteins from polar fish species are potent ice recrystallization inhibitors (IRIs) effectively stopping all ice growth. Additives that have IRI activity have been shown to enhance cellular cryopreservation with potential to improve the distribution of donor cells and tissue. Polyampholytes, polymers with both anionic and cationic side chains, are a rapidly emerging class of polymer cryoprotectants, but their mode of action and the structural features essential for activity are not clear. Here regioregular polyampholytes are synthesized from maleic anhydride copolymers to enable stoichiometric installation of the charged groups, ensuring regioregularity, which is not possible using conventional random copolymerization. A modular synthetic strategy is employed to enable the backbone and side chain hydrophobicity to be varied, with side chain hydrophobicity found to have a profound effect on the IRI activity. The activity of the regioregular polymers was found to be superior to those derived from a standard random copolymerization with statistical incorporation of monomers, demonstrating that sequence composition is crucial to the activity of IRI active polyampholytes.