Dual-modality imaging system for monitoring human heart organoids beating in vitro.
Dual-modality imaging system for monitoring human heart organoids beating in vitro.
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DOI：
10.1364/ol.493824
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发表时间：
2023-08-01
期刊：
影响因子：
3.6
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中科院分区：
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为了揭示人类心脏类器官（hHOs）的三维微观结构和钙动力学，我们开发了一种结合光学相干断层扫描（OCT）和荧光显微镜优势的双模成像系统。OCT提供高分辨率的体积结构信息，而荧光成像显示hho跳动行为的电生理学。我们验证了并发OCT运动模式（m模式）和钙成像从心脏类器官中检索到相同的跳动模式。我们进一步应用动态对比OCT （DyC-OCT）分析来加强验证和定位hho内部的跳动团簇。该成像平台为体外研究和评估hho提供了强大的工具，在疾病建模和药物筛选方面具有潜在的应用前景。
To reveal the three-dimensional microstructure and calcium dynamics of human heart organoids (hHOs), we developed a dual-modality imaging system combining the advantages of optical coherence tomography (OCT) and fluorescence microscopy. OCT provides high-resolution volumetric structural information, while fluorescence imaging indicates the electrophysiology of the hHOs’ beating behavior. We verified that concurrent OCT motion mode (M-mode) and calcium imaging retrieved the same beating pattern from the heart organoids. We further applied dynamic contrast OCT (DyC-OCT) analysis to strengthen the verification and localize the beating clusters inside the hHOs. This imaging platform provides a powerful tool for studying and assessing hHOs in vitro, with potential applications in disease modeling and drug screening.