Evaluation of autogenous PRGF plus -TCP with or without a collagen membrane on bone formation and implant osseointegration in large size bone defects. A preclinical invivo study
Evaluation of autogenous PRGF plus -TCP with or without a collagen membrane on bone formation and implant osseointegration in large size bone defects. A preclinical invivo study
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DOI：
10.1111/clr.12742
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发表时间：
2016-08-01
影响因子：
4.3
通讯作者：
Konstantinidis, Antonios
中科院分区：
文献类型：
--
作者：
Batas, Leonidas;Stavropoulos, Andreas;Konstantinidis, Antonios
ObjectivesThe的目的是评估是否连续使用的胶原蛋白膜增强骨形成和种植体骨整合在非包含的缺陷移植椅侧制备的自体富血小板生长因子（PRGF）吸附在-TCP颗粒carrier.Materials和方法大箱型缺陷（10 x6 mm; WXD）准备在无齿和完全愈合的下颌骨的6只比格犬。将具有中等粗糙表面的种植体放置在每个缺损的中心，使种植体的冠状面6 mm未被骨覆盖。剩余的缺陷空间，然后填写了椅子边制备的自体PRGF吸附在-TCP颗粒和覆盖有胶原蛋白膜（PRGF/-TCP+CM）（6缺陷）或离开没有膜（PRGF/-TCP）（5缺陷）。种植体先前暴露部分的骨整合程度各不相同，但总体上是有限的。在缺损区，PRGF/-TCP+CM组和PRGF/-TCP组的新矿化骨（%）平均值分别为43.29.86 vs. 39.9 +/- 13.7（P=0.22），相对矿化骨-种植体接触（%）平均值分别为26.2 +/- 16.45 vs. 35.91 +/- 24.45（P=0.5）。首先，从种植体顶部的骨-种植体接触是4.1 +/- 1.5和3.2 +/- 2.3（P=0.9），在PRGF/-TCP+CM和PRGF/-TCP group.ConclusionsImplantation椅子边制备的自体PRGF吸附在一个-TCP载体在非包含种植体周围的缺陷导致大量的骨再生，但骨整合是有限的。提供GBR与胶原蛋白膜没有显着提高骨再生或种植体骨整合。
ObjectivesThe aim of this study was to evaluate whether the adjunctive use of a collagen membrane enhances bone formation and implant osseointegration in non-contained defects grafted with chair-side prepared autologous platelet-rich growth factor (PRGF) adsorbed on a -TCP particulate carrier.Materials and methodsLarge box-type defects (10x6mm; WxD) were prepared in the edentulated and completely healed mandibles of six Beagles dogs. An implant with moderately rough surface was placed in the center of each defect leaving the coronal 6mm of the implant not covered with bone. The remaining defect space was then filled out with chair-side prepared autologous PRGF adsorbed on -TCP particles and either covered with a collagen membrane (PRGF/-TCP+CM) (6 defects) or left without a membrane (PRGF/-TCP) (5 defects).ResultsHistology 4months post-op showed new lamellar and woven bone formation encompassing almost entirely the defect and limited residual -TCP particles. Extent of osseointegration of the previously exposed portion of the implants varied, but in general was limited. Within the defect, new mineralized bone (%) averaged 43.29.86 vs. 39.9 +/- 13.7 in the PRGF/-TCP+CM and PRGF/-TCP group (P=0.22) and relative mineralized bone-to-implant contact (%) averaged 26.2 +/- 16.45 vs. 35.91 +/- 24.45, respectively (P=0.5). First, bone-to-implant contact from the implant top was 4.1 +/- 1.5 and 3.2 +/- 2.3 (P=0.9), in the PRGF/-TCP+CM and PRGF/-TCP group, respectively.ConclusionsImplantation of chair-side prepared autologous PRGF adsorbed on a -TCP carrier in non-contained peri-implant defects resulted in large amounts of bone regeneration, but osseointegration was limited. Provisions for GBR with a collagen membrane did not significantly enhance bone regeneration or implant osseointegration.