The Ski oncoprotein interacts with Skip, the human homolog of Drosophila Bx42
The Ski oncoprotein interacts with Skip, the human homolog of Drosophila Bx42
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DOI：
10.1038/sj.onc.1201687
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发表时间：
1998-03-26
期刊：
影响因子：
8
通讯作者：
Hayman, MJ
中科院分区：
文献类型：
--
作者：
Dahl, R;Wani, B;Hayman, MJ
v-Ski禽逆转录病毒癌基因被假定作为转录因子。由于蛋白质间相互作用在转录过程中起着重要的作用，因此我们尝试利用酵母双杂交系统来鉴定Ski蛋白的配偶体，以v-Ski序列为诱饵，（Ski相互作用蛋白）被鉴定为编码在双杂交系统中与细胞和病毒形式的Ski相互作用的蛋白，Skip与黑腹果蝇蛋白Bx 42高度同源，发现Bx 42与唾液腺的转录活性团中的染色质相关，滑雪-Skip相互作用在Ski的转化活性中具有潜在的重要性，因为Skip被证明与Ski的一个高度保守的区域相互作用，该区域是转化活性所必需的。与Ski一样，Skip在多种组织类型中表达，并定位于细胞核。
The v-Ski avian retroviral oncogene is postulated to act as a transcription factor. Since protein-protein interactions have been shown to play an important role in the transcription process, we attempted to identify Ski protein partners with the yeast two-hybrid system, Using v-Ski sequence as bait, the human gene skip (Ski Interacting Protein) was identified as encoding a protein which interacts with both the cellular and viral forms of Ski in the two-hybrid system, Skip is highly homologous to the Drosophila melanogaster protein Bx42 which is found associated with chromatin in transcriptionally active puffs of salivary glands, The Ski-Skip interaction is potentially important in Ski's transforming activity since Skip was demonstrated to interact with a highly conserved region of Ski required for transforming activity, Like Ski, Skip is expressed in multiple tissue types and is localized to the cell nucleus.