Specific changes in circulating cytokines and growth factors induced by exercise stress testing in asymptomatic aortic valve stenosis.
Specific changes in circulating cytokines and growth factors induced by exercise stress testing in asymptomatic aortic valve stenosis.
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DOI：
10.1371/journal.pone.0173787
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Undas A
中科院分区：
文献类型：
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作者：
Kolasa-Trela R;Konieczynska M;Bazanek M;Undas A
我们评估了运动引起的AS患者循环细胞因子和生长因子谱的变化。我们研究了32例连续无症状的中重度AS患者和32例年龄和性别匹配的对照组。在4个时间点（即休息时、峰值自行车运动时、限制性运动后1 h和24 h）测定血浆白细胞介素（IL）-6、IL-10、肝细胞生长因子（HGF）、血管内皮生长因子（VEGF）和转化生长因子（TGF）-β的水平。运动增加了两组的所有5项标志物（所有p<0.0001）。与对照组相比，AS组中所有测试的细胞因子的最大水平均较高（均p<0.05）。在AS患者中，VEGF、IL-6和IL-10的最高水平在运动后1小时观察到，而对照组在运动高峰时观察到。在两组中，运动后1小时观察到最大TGF- β水平。在AS组中，HGF水平在峰值时和测试后1小时较高（p = 0.0001），然而，在AS中观察到最大值在峰值时，而在对照组中在测试后。在两组中，TGF-β是运动后24小时与静息值相比保持增加的唯一标志物（p = 0.0001）。细胞因子和生长因子与心率和负荷无相关性。在无症状的中度至重度AS患者中，运动产生不同的循环细胞因子和生长因子变化模式，AS患者中所有检测细胞因子的最大水平均显著高于对照组。
We evaluated exercise-induced changes in the profile of circulating cytokines and growth factors in patients with AS. We studied 32 consecutive asymptomatic moderate-to-severe AS patients and 32 age and sex-matched controls. Plasma levels of interleukin (IL)-6, IL-10, hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), and transforming growth factor (TGF)-β were measured at 4 time points, i.e. at rest, at peak bicycle exercise, one hour and 24 hours after a symptom-limited exercise. Exercise increased all the 5 markers in both groups (all p<0.0001). The maximum levels of all tested cytokines were higher in the AS group (all p<0.05) compared with controls. In AS patients the highest levels of VEGF, IL-6, and IL-10 were observed one hour after exercise, while in the control group at peak exercise. In both groups maximum TGF- β levels were observed one hour after exercise. HGF levels were higher at peak and one hour after test in the AS group (p = 0.0001), however the maximum value in AS was observed at peak while in controls after test. In both groups TGF-β was the only marker that remained increased 24 hours after exercise compared with the value at rest (p = 0.0001). The cytokines and growth factors showed no association with heart rate and the workload. In asymptomatic patients with moderate-to-severe AS, exercise produces a different pattern of changes in circulating cytokines and growth factors, and maximum levels of all tested cytokines were significantly higher in AS patients compared with the control group.