The vertebrate homolog of the putative transforming gene of avian myelocytomatosis virus: characteristics of the DNA locus and its RNA transcript.
The vertebrate homolog of the putative transforming gene of avian myelocytomatosis virus: characteristics of the DNA locus and its RNA transcript.
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禽类骨髓细胞瘤病毒推定转化基因的脊椎动物同源物：DNA 位点及其 RNA 转录本的特征。
DOI：
10.1016/0042-6822(80)90042-2
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发表时间：
1980
期刊：
影响因子：
3.7
通讯作者：
Bishop,JM
中科院分区：
文献类型：
--
作者：
Sheiness,DK;Hughes,SH;Varmus,HE;Stubblefield,E;Bishop,JM
脊椎动物基因组含有与至少几种禽类逆转录病毒的推定转化基因同源的核苷酸序列。这些同源物中的每一种在脊椎动物中都是高度保守的;每一种都可能在正常细胞生长和发育过程中发挥作用;每一种都可能充当病毒致癌基因的祖先。本通讯证实和扩大后，这些结论提供了进一步的特性ofproto-oncMCV，细胞同源物的推定转化基因的禽骨髓细胞瘤病毒（MC 29 V）。我们使用了三种实验方法来表征原型oncMCV。首先，我们用限制性内切酶对18只鸡的原癌基因MCVin DNA进行了分析。我们的研究结果表明，原癌MCV是位于一个单一的恒定位点的鸡基因组。相比之下，携带内源性逆转录病毒基因的基因座的数量和位置从一只鸡到另一只鸡是不同的（Hughoval.，1979年a）。第二，我们用带速离心法分离鸡的染色体，发现原癌MCV与禽肉瘤病毒转化基因的细胞同源物原src不连锁。第三，我们证明了原癌MCV的主要转录物是一个2.8 kb的多聚腺苷酸化RNA，主要位于鸡成纤维细胞的细胞质中。其他鸟类的胚胎RNA也含有一个2.8kb的RNA，与原oncMCV具有同源性。我们的结论是，原oncMCV，像原src，是一个正常的基因座在脊椎动物基因组中的表达可能是必不可少的细胞生长和/或发育。
Vertebrate genomes contain nucleotide sequences homologous to the putative transforming genes of at least several avian retroviruses. Each of these homologs is highly conserved throughout vertebrates; each may function during the course of normal cellular growth and development; and each may have served as a progenitor for a viral oncogene. The present communication substantiates and enlarges upon these conclusions by providing further characterization ofproto-oncMCV, the cellular homolog of the putative transforming gene of avian myelocytomatosis virus (MC29V). We used three experimental approaches to characterizeproto-oncMCV. First, we analyzedproto-oncMCVin DNA from each of 18 chickens by using restriction endonucleases. Our results suggest thatproto-oncMCVis situated at a single constant locus in the chicken genome. By contrast, the number and location of loci harboring endogenous retrovirus genes varies from one chicken to another (Hugheset al., 1979a). Second, we fractionated chicken chromosomes by rate-zonal centrifugation and found thatproto-oncMCVis not linked toproto-src, the cellular homolog of the transforming gene of avian sarcoma virus. Third, we demonstrated that the principal transcript fromproto-oncMCVis a 2.8-kb polyadenylated RNA located primarily in the cytoplasm of chick fibroblasts. Embryonic RNA from other avian species also contained a 2.8-kb RNA that shared homology withproto-oncMCV. We conclude thatproto-oncMCV, likeproto-src, is a normal locus in the vertebrate genome whose expression may be essential to cellular growth and/or development.