Protocol to establish a lung adenocarcinoma immunotherapy allograft mouse model with FACS and immunofluorescence-based analysis of tumor response.
Protocol to establish a lung adenocarcinoma immunotherapy allograft mouse model with FACS and immunofluorescence-based analysis of tumor response.
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使用 FACS 和基于免疫荧光的肿瘤反应分析建立肺腺癌免疫治疗同种异体移植小鼠模型的方案
DOI：
10.1016/j.xpro.2021.100595
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发表时间：
2021-06-18
期刊：
影响因子：
--
通讯作者：
Sun S
中科院分区：
文献类型：
--
作者：
Peng Z;Yu M;Lin J;Dong T;Zhang X;Shi M;Qin M;Li S;Guo W;Zhang H;Sun S
抗PD-1/PD-L1治疗在许多癌症类型中显示出长期疗效，但耐药和复发仍然是该治疗的主要限制因素。在这里，我们描述了一种在小鼠肺癌模型中评估免疫治疗对肿瘤反应的方案。该方案包括肺癌小鼠模型的建立、抗PD-1治疗、肿瘤浸润性淋巴细胞分离、免疫荧光和流式细胞术分析。该方案也可应用于其他癌症类型和免疫疗法。有关该方案的使用和执行的完整细节，请参阅从基因工程肺癌小鼠模型建立肿瘤衍生细胞系使用同种异体肺癌模型评估肿瘤对免疫治疗的反应从新鲜肿瘤样本中分离肿瘤浸润性淋巴细胞通过流式细胞仪和免疫荧光检测淋巴细胞活性抗PD-1/PD-L1疗法在许多癌症类型中显示出长期疗效，但耐药和复发仍然是该疗法的主要限制因素。在这里，我们描述了一种在小鼠肺癌模型中评估免疫治疗对肿瘤反应的方案。该方案包括肺癌小鼠模型的建立、抗PD-1治疗、肿瘤浸润性淋巴细胞分离、免疫荧光和流式细胞术分析。该方案也可应用于其他癌症类型和免疫疗法。
Anti-PD-1/PD-L1 therapy shows long-term effects in many cancer types, but resistance and relapse remain the main limitations of this therapy. Here, we describe a protocol to evaluate the tumor response to immunotherapy in a mouse lung cancer model. The protocol includes the establishment of the lung cancer mouse model, anti-PD-1 treatment, tumor-infiltrating lymphocyte isolation, immunofluorescence, and flow cytometry analysis. This protocol can also be applied to other cancer types and immunotherapies. For complete details on the use and execution of this protocol, please refer to Establish a tumor-derived cell line from a genetic engineered lung cancer mouse model Evaluate the tumor response to immunotherapy using an allograft lung cancer model Isolate tumor-infiltrating lymphocytes from fresh tumor samples Evaluate activity of lymphocytes by flow cytometry and infiltration by immunofluorescence Anti-PD-1/PD-L1 therapy shows long-term effects in many cancer types, but resistance and relapse remain the main limitations of this therapy. Here, we describe a protocol to evaluate the tumor response to immunotherapy in a mouse lung cancer model. The protocol includes the establishment of the lung cancer mouse model, anti-PD-1 treatment, tumor-infiltrating lymphocyte isolation, immunofluorescence, and flow cytometry analysis. This protocol can also be applied to other cancer types and immunotherapies.
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影响因子：
32.4
作者：
Joshi NS;Akama-Garren EH;Lu Y;Lee DY;Chang GP;Li A;DuPage M;Tammela T;Kerper NR;Farago AF;Robbins R;Crowley DM;Bronson RT;Jacks T
通讯作者：
Jacks T
影响因子：
16
作者：
Yu, Man;Peng, Zhengxin;Sun, Shuguo
通讯作者：
Sun, Shuguo
影响因子：
50.3
作者：
DuPage M;Cheung AF;Mazumdar C;Winslow MM;Bronson R;Schmidt LM;Crowley D;Chen J;Jacks T
通讯作者：
Jacks T
影响因子：
64.8
作者：
Liu Z;Gerner MY;Van Panhuys N;Levine AG;Rudensky AY;Germain RN
通讯作者：
Germain RN