Ginkgolide B Suppresses Intercellular Adhesion Molecule-1 Expression via Blocking Nuclear Factor-κB Activation in Human Vascular Endothelial Cells Stimulated by Oxidized Low-Density Lipoprotein
Ginkgolide B Suppresses Intercellular Adhesion Molecule-1 Expression via Blocking Nuclear Factor-κB Activation in Human Vascular Endothelial Cells Stimulated by Oxidized Low-Density Lipoprotein
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DOI：
10.1254/jphs.08275fp
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发表时间：
2009-07-01
影响因子：
3.5
通讯作者：
Qi, Ruomei
中科院分区：
文献类型：
--
作者：
Li, Rui;Chen, Beidong;Qi, Ruomei
动脉粥样硬化是一种复杂的动脉炎性疾病。氧化低密度脂蛋白（ox-LDL）与慢性血管炎症直接相关。在本研究中，我们测试的假设，银杏内酯B，一个传统的中药成分的心脏疾病，可能会影响ox-LDL诱导的炎症反应在人脐静脉内皮细胞（HUVECs）。结果显示，ox-LDL处理后，HUVECs细胞间粘附分子-1（ICAM-1）表达明显增加，这与I κ B磷酸化水平显著增加和核因子-κ B（NF-κ B）向核内迁移有关。有趣的是，银杏内酯B可以减弱ox-LDL诱导的ICAM-1表达和NF-κ B的重新定位。此外，银杏内酯B显着减少ox-LDL诱导的活性氧（ROS）的产生。银杏内酯B可能通过阻断NF-κ B B信号通路和抑制ROS的产生，减轻ox-LDL诱导的HUVECs炎症反应。
Atherosclerosis is a complex inflammatory arterial disease. Oxidized low-density lipoprotein (ox-LDL) is directly associated with chronic vascular inflammation. In the current study, we tested the hypothesis that ginkgolide B, a component of traditional Chinese herbal medicine for heart disorder, may affect ox-LDL-induced inflammatory responses in human umbilical vein endothelial cells (HUVECs). The results showed that the ox-LDL treatment caused a significantly increase in the expression of intercellular adhesion molecule-1 (ICAM-1) in HUVECs, which was associated with a dramatic augmentation in phosphorylation Of I kappa B and relocation of nuclear factor-kappa B (NF-kappa B) into the nuclei. Interestingly, the ox-LDL-induced ICAM-1 expression and NF-kappa B relocation could be attenuated by addition of ginkgolide B. Moreover, ginkgolide B significantly reduces ox-LDL-induced generation of reactive oxygen species (ROS). In conclusion, ginkgolide B may decrease inflammatory responses induced by ox-LDL via blocking NF-kappa B signaling and inhibiting ROS generation in HUVECs.