Calcium-binding proteins and calcium-release channels in human maturing oocytes, pronuclear zygotes and early preimplantation embryos
Calcium-binding proteins and calcium-release channels in human maturing oocytes, pronuclear zygotes and early preimplantation embryos
复制标题
DOI：
10.1093/humrep/17.11.2938
复制
发表时间：
2002-11-01
影响因子：
6.1
通讯作者：
Opas, M
中科院分区：
文献类型：
--
作者：
Balakier, H;Dziak, E;Opas, M
背景：研究目的是研究Ca2+结合蛋白和Ca2+释放受体通道在人成熟卵母细胞、原核受精卵和植入前胚胎中的存在和定位。方法：对人卵母细胞和胚胎进行免疫细胞化学分析，使用针对所研究蛋白质的特异性抗体，然后使用共聚焦激光显微镜。结果：在所有细胞类型中均检测到钙网蛋白（Calreticulin）和钙固蛋白（calsequestrin），三磷酸肌醇和红嘌呤受体（InsP(3)R-2、RyRs-1、2、3）两种钙释放受体，以及分子伴侣钙连联蛋白（calnexin）。钙网蛋白主要分布在细胞皮层和核膜中，钙钉蛋白分布在整个细胞质中。一般来说，InsP(3)R-2和RyRs的定位分别与calreticulin和calsequestrin相似。两种受体均富集于减数分裂卵母细胞的质下区。此外，在卵母细胞和卵裂球的核结构中检测到InsP(3)R。钙连蛋白的分布与钙网蛋白重叠，但似乎存在于不同的亚室中。结论：人卵母细胞和胚胎以高度有组织和发育调节的方式表达钙的吸收和释放蛋白。这些蛋白的微调可能在卵母细胞成熟、受精和早期胚胎发育过程中对Ca2+短暂性的调节中起关键作用。
BACKGROUND: The study aim was to investigate the presence and localization of Ca2+-binding proteins and Ca2+-release receptor channels in human maturing oocytes, pronuclear zygotes and preimplantation embryos. METHODS: Immunocytochemical analysis, using specific antibodies against the proteins being studied, followed with confocal laser microscopy, was performed on human oocytes and embryos. RESULTS: Calreticulin and calsequestrin (the two major calcium storage proteins of somatic cells), two types of calcium release receptors, the inositol trisphosphate and ryanodine receptors (InsP(3)R-2, RyRs-1,2,3), and the molecular chaperone, calnexin, were identified in all investigated cell types. Calreticulin was predominant in the cell cortex and in the nuclear envelope, while calsequestrin was distributed throughout the entire cytoplasm. Generally, localization of the InsP(3)R-2 and RyRs was similar to that of calreticulin and calsequestrin respectively. Both types of receptor were enriched in the subplasmalemmal region of meiotic oocytes. In addition, the InsP(3)R was detected in the nuclear structures of oocytes and blastomeres. Calnexin distribution overlapped with that of calreticulin but appeared to be present in distinct subcompartments. CONCLUSIONS: Human oocytes and embryos express the calcium sequestration and release proteins in highly organized and developmentally regulated patterns. Fine-tuning of these proteins may play a crucial role in regulation of Ca2+ transience during oocyte maturation, fertilization and early embryo development.