Mollusk shell acidic proteins: In search of individual functions
Mollusk shell acidic proteins: In search of individual functions
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DOI：
10.1002/cbic.200200548
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发表时间：
2003-06-06
期刊：
影响因子：
3.2
通讯作者：
Weiner, S
中科院分区：
文献类型：
--
作者：
Gotliv, BA;Addadi, L;Weiner, S
酸性蛋白质在生物矿化过程中起主要作用。这些蛋白质通常被认为控制矿物质的形成和生长。因此，单个酸性蛋白的表征是重要的，作为将功能与单个蛋白联系起来的第一步，这是我们的最终目标。为了表征负责生物矿物组装的蛋白质，开发了一种新的凝胶电泳固定和染色方案，并且首次在凝胶上观察到许多（如果不是全部）酸性蛋白质。在体外试验中，我们表明从文石壳层中提取的蛋白质在转化为文石晶体形式之前诱导无定形碳酸钙的形成。这项研究消除了一些主要的障碍，在酸性蛋白质的表征和揭示更多的光这些蛋白质在生物矿化过程中的功能。
Acidic proteins play a major role in the biomineralization process. These proteins are generally thought to control mineral formation and growth. Thus, characterization of individual acidic proteins is important as a first step toward linking function to individual proteins, which is our ultimate goal. In order to characterize the protein(s) responsible for the assemblage of biominerals, a new gel electrophoresis fixing and staining protocol was developed and many, if not all of the acidic proteins were visualized on the gel for the first time. In an in vitro assay we show that proteins extracted from an aragonitic shell layer induce the formation of amorphous calcium carbonate prior to its transformation into the aragonitic crystalline form. This study removes some major obstacles in the characterization of acidic proteins and sheds more light on the functions of these proteins in the biomineralization process.