Gram-positive merA gene in gram-negative oral and urine bacteria.
Gram-positive merA gene in gram-negative oral and urine bacteria.
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DOI：
10.1016/j.femsle.2004.08.004
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发表时间：
2004-09
影响因子：
2.1
通讯作者：
K. Ojo;D. Tung;H. Luís;M. Bernardo;J. Leitão;M. Roberts
中科院分区：
文献类型：
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作者：
K. Ojo;D. Tung;H. Luís;M. Bernardo;J. Leitão;M. Roberts
采用DNA-DNA杂交、PCR和测序技术对临床耐汞（Hgr）革兰氏阴性菌进行了鉴定，革兰氏阳性汞还原酶（merA）样基因。建立了一种PCR检测方法，通过PCR产物的大小差异来区分与葡萄球菌相关的merAgenes和与芽孢杆菌/链球菌相关的merAgenes。DNA序列分析与PCR试验相关。对来自junii machine - tobacter， cloacaeenterobacter和大肠杆菌的thermeragenes进行测序，发现与金黄色葡萄球菌mera蛋白具有98-99%相同的核苷酸（nt）和99.6-100%相同的氨基酸。第4个meragene来自pantoeae agglomerans，部分测序（60%），与口腔链球菌mera蛋白具有99%相同的nt和100%的氨基酸一致性。所有革兰氏阴性细菌将其革兰氏阳性细菌基因转移到革兰氏阳性粪肠球菌受体，并产生表达汞抗性的转偶联物。这些革兰氏阳性基因与革兰氏阳性四环素耐药基因和革兰氏阳性大环内酯耐药基因一起，与能够在革兰氏阴性细菌中转移和表达的移动元件相关联。
Clinical mercury resistant (Hgr) Gram-negative bacteria carrying Gram-positive mercury reductase (merA)-like genes were characterized using DNA-DNA hybridization, PCR and sequencing. A PCR assay was developed which discriminated between themerAgenes related toStaphylococcusand those related to theBacillus/Streptococcus merAgenes by the difference in size of the PCR product. DNA sequence analysis correlated with the PCR assay. ThemerAgenes fromAcinetobacter junii, Enterobacter cloacaeandEscherichia coliwere sequenced and shared 98–99% identical nucleotide (nt) and 99.6–100% amino acid identity with theStaphylococcus aureusMerA protein. A fourthmerAgene, fromPantoeae agglomerans, was partially sequenced (60%) and had 99% identical nt and 100% amino acid identity with theStreptococcus oralisMerA protein. All the HgrGram-negative bacteria transferred their Gram-positivemerAgenes to a Gram-positiveEnterococcus faecalisrecipient with the resulting transconjugants expressing mercury resistance. These Gram-positivemerAgenes join Gram-positive tetracycline resistance and Gram-positive macrolide resistance genes in their association with mobile elements which are able to transfer and express in Gram-negative bacteria.