Generation of human-induced pluripotent stem cells from burn patient-derived skin fibroblasts using a non-integrative method.
Generation of human-induced pluripotent stem cells from burn patient-derived skin fibroblasts using a non-integrative method.
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使用非整合方法从烧伤患者来源的皮肤成纤维细胞生成人诱导多能干细胞
DOI：
10.3892/ijmm.2017.3206
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发表时间：
2018-01
影响因子：
5.4
通讯作者：
Liu D
中科院分区：
文献类型：
--
作者：
Fu S;Ding J;Liu D;Huang H;Li M;Liu Y;Tu L;Liu D
患者特异性诱导多能干细胞（iPSCs）已被认为是皮肤组织工程中可能的细胞来源。它们有可能使大面积皮肤烧伤或皮肤缺陷的患者受益匪浅。然而，基于整合病毒的重编程方法具有较高的基因突变风险，并且小鼠胚胎成纤维细胞饲养细胞可能是一种污染物。在本研究中，我们成功地从烧伤患者身上获取了人皮肤成纤维细胞（hsf），并使用无喂食的非整合方法生成了患者特异性的iPSCs。用仙台病毒载体传递八聚体结合转录因子4 （OCT4）、性别决定区Y盒2 （SOX2）和NANOG转录因子。iPSCs具有典型的人胚胎干细胞样形态和增殖特征。它们也表达多能性标记，包括OCT4、NANOG、SOX2、TRA181、阶段特异性胚胎抗原4和TRA-160，并表现出正常的核型。畸胎瘤和胚胎样体的形成表明，iPSCs能够在体外和体内分化为所有三种胚层的细胞。本研究结果表明，来自烧伤患者的hsf可能被重编程为具有多能性的干细胞，这为未来基于细胞的皮肤组织工程提供了基础。
Patient specific induced pluripotent stem cells (iPSCs) have been recognized as a possible source of cells for skin tissue engineering. They have the potential to greatly benefit patients with large areas of burned skin or skin defects. However, the integration virus-based reprogramming method is associated with a high risk of genetic mutation and mouse embryonic fibroblast feeder-cells may be a pollutant. In the present study, human skin fibroblasts (HSFs) were successfully harvested from patients with burns and patient-specific iPSCs were generated using a non-integration method with a feeder-free approach. The octamer-binding transcription factor 4 (OCT4), sex-determining region Y box 2 (SOX2) and NANOG transcription factors were delivered using Sendai virus vectors. iPSCs exhibited representative human embryonic stem cell-like morphology and proliferation characteristics. They also expressed pluripotent markers, including OCT4, NANOG, SOX2, TRA181, stage-specific embryonic antigen 4 and TRA-160, and exhibited a normal karyotype. Teratoma and embryoid body formation revealed that iPSCs were able to differentiate into cells of all three germ layers in vitro and in vivo. The results of the present study demonstrate that HSFs derived from patients with burns, may be reprogrammed into stem cells with pluripotency, which provides a basis for cell-based skin tissue engineering in the future.
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DOI：
10.1007/978-1-62703-511-8_7
发表时间：
2013
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Churko JM;Burridge PW;Wu JC
通讯作者：
Wu JC
影响因子：
64.5
作者：
Takahashi, Kazutoshi;Yamanaka, Shinya
通讯作者：
Yamanaka, Shinya
影响因子：
3.7
作者：
Ohta S;Imaizumi Y;Okada Y;Akamatsu W;Kuwahara R;Ohyama M;Amagai M;Matsuzaki Y;Yamanaka S;Okano H;Kawakami Y
通讯作者：
Kawakami Y
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
5.9
作者：
Ando M;Nishimura T;Yamazaki S;Yamaguchi T;Kawana-Tachikawa A;Hayama T;Nakauchi Y;Ando J;Ota Y;Takahashi S;Nishimura K;Ohtaka M;Nakanishi M;Miles JJ;Burrows SR;Brenner MK;Nakauchi H
通讯作者：
Nakauchi H