A Phenol-Chloroform Protocol for Extracting DNA from Ancient Samples
A Phenol-Chloroform Protocol for Extracting DNA from Ancient Samples
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DOI：
10.1007/978-1-61779-516-9_2
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发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Larson, Greger
中科院分区：
文献类型：
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作者：
Barnett, Ross;Larson, Greger
古代DNA（aDNA）分析的无数下游应用最终都需要从古代材料的提取物中产生序列数据。从已知含有保存的生物分子的组织（例如牙齿、毛发、组织、骨骼）中提取DNA依赖于对已经使用了近二十年的基本技术的细微修改。已经引入了多种DNA提取方案，根据组织类型和古代组织所暴露的长期保存环境，成功程度不同。在这里，我们描述了从任何组织类型中提取aDNA的酚-氯仿方法。这种常用的方法允许回收总核酸含量，同时低分子量双链DNA的损失最小。
The myriad downstream applications of ancient DNA (aDNA) analysis all ultimately require that sequence data are generated from extracts of ancient material. DNA extraction from tissues known to contain preserved biomolecules (e.g. teeth, hair, tissue, bone) relies on subtle modifications of a basic technique that has been in use for nearly two decades. Multiple DNA extraction protocols have been introduced, with varying levels of success depending on tissue type and the long-term preservation environment to which the ancient tissue was exposed. Here, we describe the phenol-chloroform method for extracting aDNA from any tissue type. This commonly employed method allows for the recovery of total nucleic acid content with minimal loss of low molecular weight double-stranded DNA.