The yeast lysyl-tRNA synthetase gene. Evidence for general amino acid control of its expression and domain structure of the encoded protein.
The yeast lysyl-tRNA synthetase gene. Evidence for general amino acid control of its expression and domain structure of the encoded protein.
复制标题
酵母赖氨酰-tRNA 合成酶基因。
DOI：
--
复制
发表时间：
1988
影响因子：
4.8
通讯作者：
Jean
中科院分区：
文献类型：
--
作者：
Marc MirandeS;Jean
测定了酵母胞质赖氨酰-tRNA合成酶(KRS1)结构基因及其侧翼区的3.6kb碱基DNA片段的核苷酸序列。编码的67,881 kDa的蛋白质在其氨基末端的29个氨基酸残基片段中显示了由11个赖氨酸组成的簇。有证据表明，这个片段是天然酶对多阴离子载体表现出的亲和力的原因。确定了KRS1基因的转录起始点。从TATA盒的上游，鉴定了与RPG盒和一般氨基酸控制系统的连接序列相对应的假定控制元件。证据表明，KRS1基因的转录诱导通过一般的氨基酸控制系统。
The nucleotide sequence of a 3.6-kilobase pair DNA fragment containing the structural gene for yeast cytoplasmic lysyl-tRNA synthetase (KRS1) and its flanking regions was determined. The encoded protein of 67,881 kDa displays a cluster of 11 lysines within a 29-amino acid residue segment at its amino-terminal extremity. Evidence is presented that this segment is responsible for the affinity displayed by the native enzyme toward polyanionic carriers. The transcription initiation sites of the KRS1 gene were determined. Upstream from the TATA box, putative control elements corresponding to the concensus sequences for the RPG box and the general amino acid control system were identified. Evidence for transcriptional induction of the KRS1 gene via the general amino acid control system is presented.