Tissue Response to a Porous Collagen Matrix Used for Soft Tissue Augmentation
Tissue Response to a Porous Collagen Matrix Used for Soft Tissue Augmentation
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DOI：
10.3390/ma12223721
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发表时间：
2019-11-02
期刊：
影响因子：
3.4
通讯作者：
Bosshardt, Dieter D.
中科院分区：
文献类型：
--
作者：
Caballe-Serrano, Jordi;Zhang, Sophia;Bosshardt, Dieter D.
短的炎症期和血管和间充质细胞的快速向内生长对于生物材料的组织整合是必不可少的。宏观经济在这一过程中发挥着关键作用。我们研究了巨噬细胞、血管和增殖细胞侵入高度多孔且体积稳定的胶原基质（VCMX），该基质用于牙齿和牙科植入物周围的软组织增强。将生物材料植入犬上颌骨的粘膜下囊中，并在6个不同的时间点分析组织反应。对增殖细胞（PCNA）、巨噬细胞（MAC387）、多核巨细胞（CD86）和血管（TGM2）进行免疫组织化学。血液迅速充满VCMX毛孔。在第一周，MAC387+细胞填充VCMX孔，血管和PCNA+细胞侵入VCMX，并观察到散在的CD86+细胞。第15天，MAC387+细胞稀少，血管已完全侵入VCMX，增殖细胞数量达到高峰，并出现成纤维细胞。在30天时，MAC387+不存在，增殖和CD86+细胞的数量下降，而血管和成纤维细胞的数量较高。在90天时，残留的VCMX在软结缔组织中整合良好。总之，VCMX引发了一个短暂的炎症阶段，然后是快速的组织整合。
A short inflammatory phase and fast ingrowth of blood vessels and mesenchymal cells are essential for tissue integration of a biomaterial. Macrophages play a key role in this process. We investigated invasion of macrophages, blood vessels, and proliferating cells into a highly porous and volume-stable collagen matrix (VCMX) used for soft tissue augmentation around teeth and dental implants. The biomaterial was implanted in submucosal pouches in the canine maxilla, and the tissue response was analyzed at six different time points. Immunohistochemistry was done for proliferating cells (PCNA), macrophages (MAC387), multinucleated giant cells (CD86), and blood vessels (TGM2). Blood rapidly filled the VCMX pores. During the first week, MAC387+ cells populated the VCMX pores, blood vessels and PCNA+ cells invaded the VCMX, and CD86+ scattered cells were observed. At 15 days, MAC387+ cells were scanty, blood vessels had completely invaded the VCMX, the number of proliferating cells peaked, and fibroblasts appeared. At 30 days, MAC387+ were absent, the numbers of proliferating and CD86+ cells had declined, while blood vessel and fibroblast numbers were high. At 90 days, residual VCMX was well-integrated in soft connective tissue. In conclusion, the VCMX elicited a short inflammatory phase followed by rapid tissue integration.