Facile synthesis of boronic acid-functionalized magnetic carbon nanotubes for highly specific enrichment of glycopeptides
Facile synthesis of boronic acid-functionalized magnetic carbon nanotubes for highly specific enrichment of glycopeptides
复制标题
轻松合成硼酸功能化磁性碳纳米管，用于高度特异性富集糖肽
DOI：
10.1039/c3nr05367a
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发表时间：
2014-01-01
期刊：
影响因子：
6.7
通讯作者：
Ju, Huangxian
中科院分区：
文献类型：
--
作者：
Ma, Rongna;Hu, Junjie;Ju, Huangxian
采用分步合成法制备了硼酸功能化磁性碳纳米管（MCNT），用于糖肽的高特异性富集。通过溶剂热反应将Fe 3+负载在酸处理的碳纳米管上，并与1-芘丁酸N-羟基琥珀酰亚胺酯（PASE）通过酰胺化反应结合氨基苯硼酸（APBA），制备了MCNTs。PASE的引入可以将MCNT和APBA连接在一起，抑制其非特异性吸附，降低结合分子间的空间位阻。由于MCNTs的优异结构，PASE然后APBA在MCNTs上的功能化是相当简单、特异和有效的。利用APBA-MCNTs的硼基与糖肽的可逆共价结合，实现了磁场对糖肽的富集和分离。特别大的比表面积和高密度的硼酸基团的APBA-MCNTs导致快速和高效的富集糖肽，即使在存在大量的干扰nonglycopeptides。功能MCNT具有高选择性富集的21个糖肽从辣根过氧化物酶的消化证明MALDI-TOF质谱分析显示更多的糖肽检测比通常的9个糖肽与市售的APBA-琼脂糖。所提出的系统显示出更好的特异性，即使在非糖肽的浓度高出50倍。硼酸功能化的MCNT为精确的糖蛋白质组学分析提供了一个有前途的选择性富集平台。
A stepwise strategy was developed to synthesize boronic acid functionalized magnetic carbon nanotubes (MCNTs) for highly specific enrichment of glycopeptides. The MCNTs were synthesized by a solvothermal reaction of Fe3+ loaded on the acid-treated CNTs and modified with 1-pyrenebutanoic acid N-hydroxysuccinimidyl ester (PASE) to bind aminophenylboronic acid (APBA) via an amide reaction. The introduction of PASE could bridge the MCNT and APBA, suppress the nonspecific adsorption and reduce the steric hindrance among the bound molecules. Due to the excellent structure of the MCNTs, the functionalization of PASE and then APBA on MCNTs was quite simple, specific and effective. The glycopeptides enrichment and separation with a magnetic field could be achieved by their reversible covalent binding with the boronic group of APBA-MCNTs. The exceptionally large specific surface area and the high density of boronic acid groups of APBA-MCNTs resulted in rapid and highly efficient enrichment of glycopeptides, even in the presence of large amounts of interfering nonglycopeptides. The functional MCNTs possessed high selectivity for enrichment of 21 glycopeptides from the digest of horseradish peroxidase demonstrated by MALDI-TOF mass spectrometric analysis showing more glycopeptides detected than the usual 9 glycopeptides with commercially available APBA-agarose. The proposed system showed better specificity for glycopeptides even in the presence of non-glycopeptides with 50 times higher concentration. The boronic acid functionalized MCNTs provide a promising selective enrichment platform for precise glycoproteomic analysis.