Cytochrome P-450 monooxygenase systems in aquatic species: carcinogen metabolism and biomarkers for carcinogen and pollutant exposure.
Cytochrome P-450 monooxygenase systems in aquatic species: carcinogen metabolism and biomarkers for carcinogen and pollutant exposure.
复制标题
DOI：
10.1289/ehp.90-1519513
复制
发表时间：
1991-01
影响因子：
10.4
通讯作者：
中科院分区：
文献类型：
--
作者：
高水平的多环芳烃（PAH）致癌物通常发生在水生系统中，鱼类和其他动物的肿瘤出现。转化多环芳烃的酶可以引发这些疾病，并可以指示致癌物和其他污染物对鱼类的污染。细胞色素P-450在激活鱼类和哺乳动物物种中的PAH致癌物方面具有类似的作用。多环芳烃和许多氯化烃，例如，多氯联苯（PCBs）在鱼类体内诱导一种细胞色素P-450，这是多环芳烃代谢的主要催化剂。这种P-450在鱼类中的诱导可以加速碳氢化合物的处置，但也可以促进多环芳烃致癌衍生物的形成。无脊椎动物的PAH代谢率低于鱼类。这些利率是不明显的诱导暴露于多环芳烃或多氯联苯。较低的外来化合物代谢率导致双壳类软体动物（蛤、贻贝等）中污染物残留水平较高。这可能会限制某些原致癌物（需要活化）参与无脊椎动物的疾病过程。P-450的诱导形式可以表明鱼类暴露于多环芳烃，多氯联苯和其他有毒化合物。这不仅限于致癌物质。通过使用催化分析、鱼类P-450抗体和与P-450信使RNA杂交的cDNA探针，在污染区的鱼类中检测到环境诱导。这些方法的应用可以提供灵敏的生物监测工具，可以检测环境中某些致癌物和肿瘤促进剂对鱼类的污染。（250字处删节）
High levels of polynuclear aromatic hydrocarbon (PAH) carcinogens commonly occur in aquatic systems where neoplasms arise in fish and other animals. Enzymes that transform PAHs can act in initiating these diseases and can indicate the contamination of fish by carcinogens and other pollutants. Cytochrome P-450 has similar roles in activating PAH carcinogens in fish and mammalian species. PAHs and many chlorinated hydrocarbons, e.g., polychlorinated biphenyls (PCBs) induce a form of cytochrome P-450 in fish that is the primary catalyst of PAH metabolism. The induction of this P-450 in fish can accelerate the disposition of hydrocarbons, but can also enhance the formation of carcinogenic derivatives of PAHs. Invertebrates have lower rates of PAH metabolism than fish. These rates are not obviously inducible by exposure to PAHs or PCBs. The lower rates of foreign compound metabolism contribute to higher pollutant residue levels in bivalve mollusks (clams, mussels, etc.) than in fish and may limit the involvement of some procarcinogens (requiring activation) in disease processes in invertebrates. The induction of P-450 forms can indicate the exposure of fish to PAHs, PCBs, and other toxic compounds. This is not restricted to carcinogens. Environmental induction has been detected in fish from contaminated areas by use of catalytic assay, antibodies to fish P-450, and cDNA probes that hybridize with P-450 messenger RNA. Application of these methods can provide sensitive biological monitoring tools that can detect environmental contamination of fish by some carcinogens and tumor promoters.(ABSTRACT TRUNCATED AT 250 WORDS)