Brazilian propolis (AF-08) inhibits collagen-induced platelet aggregation without affecting blood coagulation
Brazilian propolis (AF-08) inhibits collagen-induced platelet aggregation without affecting blood coagulation
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DOI：
10.1007/s11418-021-01518-w
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发表时间：
2021-05
影响因子：
3.3
通讯作者：
C. Sugita;A. Yamashita;Shigetoshi Tsutsumi;H. Kai;T. Sonoda;H. Yoshida;Ryuichi Yamamoto;Y. Asada;M. Kurokawa
中科院分区：
文献类型：
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作者：
C. Sugita;A. Yamashita;Shigetoshi Tsutsumi;H. Kai;T. Sonoda;H. Yoshida;Ryuichi Yamamoto;Y. Asada;M. Kurokawa
巴西芦荟（AF-08）是一种含有多种黄酮类化合物的膳食补充剂。它在世界范围内被用作民间药物。类黄酮和含有它们的水果和蔬菜饮食已被证明可以降低心血管疾病（CVD）的风险。大多数心血管疾病是由动脉血栓形成引起的。血栓是由血小板与受损血管的粘附和聚集以及由外源性和内源性途径组成的血液凝固之间的相互作用形成的。血小板聚集和血液凝固与血栓形成密切相关。因此，我们通过检测AF-08或其组分黄酮类化合物对血小板聚集和血液凝固的抑制来评估其抗血栓形成的有效性。将人富血小板血浆与系列稀释的AF-08孵育10分钟，以评估其对胶原引起的血小板聚集的抑制作用。通过凝血酶原时间（PT）和活化部分凝血活酶时间（APTT）评价AF-08对凝血功能的抑制作用，PT和APTT分别反映了外源性和内源性途径的凝血功能。AF-08显着抑制胶原诱导的血小板聚集，但不抑制PT和APTT，表明AF-08抑制血小板聚集，但不抑制血液凝固。AF-08中的3种黄酮类化合物中，芹菜素和白杨素对血小板聚集有明显的抑制作用，山奈酚的抑制作用较弱。三种黄酮类化合物对PT和APTT无影响。AF-08对人血小板聚集的抑制活性而不影响血液凝固，这表明部分是由于芹菜素和白杨素。AF-08可能有效抑制血小板为基础的动脉血栓形成，降低心血管疾病的风险。
Brazilian propolis (AF-08) is a dietary supplement containing a variety of flavonoids. It is used worldwide as a folk medicine. Flavonoids and a diet of fruits and vegetables containing them have been shown to reduce the risk of cardiovascular diseases (CVDs). Most of CVDs are caused by arterial thrombus formation. A thrombus is formed by the interaction between adhesion and aggregation of platelets to damaged blood vessels and blood coagulation consisting of extrisic and intrinsic pathways. Platelet aggregation and blood coagulation are closely linked to thrombosis. Therefore, we evaluated the effectiveness of AF-08 or its component flavonoids against thrombosis by examining their inhibition of platelet aggregation and blood coagulation. Human platelet-rich plasma was incubated with serial dilutions of AF-08 for 10 min to assess its inhibitory effect on platelet aggregation caused by collagen. The inhibitory effect of AF-08 on blood coagulation was evaluated by the prothrombin time (PT) and activated partial thromboplastin time (APTT), which reflect the coagulation function of extrinsic and intrinsic pathways, respectively. AF-08 significantly inhibited collagen-induced platelet aggregation but not PT and APTT, indicating that AF-08 inhibited platelet aggregation but not blood coagulation. Among three flavonoids contained in AF-08, apigenin and chrysin obviously inhibited platelet aggregation but the inhibitory effect of kaempferol was less effective. The three flavonoids did not affect PT and APTT. The inhibitory activity of AF-08 on human platelet aggregation without affecting blood coagulation was suggested to be partially due to apigenin and chrysin. AF-08 may be effective in suppressing platelet-based arterial thrombus formation and reducing the risk of CVDs.