Androgen receptors in ventral prostate glands of zinc deficient rats.
Androgen receptors in ventral prostate glands of zinc deficient rats.
复制标题
缺锌大鼠腹侧前列腺中的雄激素受体。
DOI：
10.1016/0024-3205(86)90082-2
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发表时间：
1986
期刊：
影响因子：
6.1
通讯作者：
Rennert,OM
中科院分区：
文献类型：
--
作者：
Chung,KW;Kim,SY;Chan,WY;Rennert,OM
用炭法研究了锌缺乏大鼠前列腺细胞质中雄激素的结合。将大鼠单独饲养在塑料笼中，并维持3个月的缺锌饮食。用不同浓度的氚化甲基三烯诺酮（3H-R1881，一种合成雄激素）单独或在放射性惰性物质R1881的500倍过量存在下孵育前列腺细胞质部分。Scatchard分析结果显示，锌缺乏大鼠前列腺细胞质中雄激素结合位点的数量为31±5.2 fmol/mg。这明显低于对照组（84±11.5 fmol/mg蛋白）。另一方面，它们的解离常数（Kd = 1.6±0.6 nM）与对照动物（1.7±0.7 nM）无显著差异。在缺锌状态下，胞浆受体位点浓度的降低表明这种金属参与了靶细胞中的雄激素结合过程。
Androgen binding has been studied in the prostate cytosol of zinc deficient rats by charcoal assays. Rats were housed individually in plastic cages and maintained on a zinc deficient diet for 3 months. The cytosol fraction of prostate gland was incubated with various concentrations of tritiated methyltrienolone (3H-R1881, a synthetic androgen) alone or in the presence of 500-fold excess of radioinert R1881. Scatchard analysis of the data revealed that the number of androgen binding sites in the cytosol fraction of the zinc deficient rat prostate was 31 ± 5.2 fmol/mg cytosol protein. This was significantly lower than that (84 ± 11.5 fmol/mg protein) of the controls. Their dissociation constant (Kd = 1.6 ± 0.6 nM) on the other hand was not different from that (1.7 ± 0.7 nM) of control animals. This decrease in the concentration of cytosol receptor sites in the zinc deficient state suggests that this metal is involved in the androgen-binding process in the target cells.