Comparative genomic hybridization study of nasal-type NK/T-cell lymphoma
Comparative genomic hybridization study of nasal-type NK/T-cell lymphoma
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DOI：
10.1002/cyto.1069
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发表时间：
2001-04-15
期刊：
影响因子：
--
通讯作者：
Ree, HJ
中科院分区：
文献类型：
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作者：
Ko, YH;Choi, KE;Ree, HJ
背景资料：鼻型NWT细胞淋巴瘤是一种罕见的非霍奇金淋巴瘤，其遗传学改变与发病机制尚未明确，本研究探讨鼻型NWT细胞淋巴瘤的非随机遗传学改变。本文对9例鼻型NWT细胞淋巴瘤进行了比较基因组杂交（CGH），应用聚合酶链反应-单链构象多态性分析（PCR-SSCP）技术分析6 q、1 p和17 p染色体杂合性丢失（洛）和p53基因突变。结果如下：7例鼻型NWT细胞淋巴瘤的DNA拷贝数变化为染色体2 q（5）、13 q（4）、10 q（3）、21 q（2）、3q（2）、5 q（2）和17 q（2）增加，染色体1 p（4）、17 p（4）、12 q（3）、13 q（2）和6 q（1）丢失，6例6 q至少有2个标记的病例中，1例在D 6S 300、D 6S 1639、D 6S 261、D 6S 407和D 6S 292位点出现洛，2例1 p和17 q缺失的病例在D1 S214、D1 S503位点出现洛，D17S559结论：NWT细胞淋巴瘤1 p、17 p、12 q DNA缺失和2 q、13 q、10 q DNA增加的频率较高，提示这些区域可作为进一步研究肿瘤发生相关抑癌基因或癌基因的分子遗传学靶点。与以往研究相反，6 q的罕见改变引起了人们对6 q缺失在早期NWT细胞淋巴瘤发病机制中的意义的怀疑，需要对更明确的病例进行进一步研究以验证其相关性。(C)2001 Wiley-Liss，Inc.
Background: Nasal-type NWT-cell lymphoma is a rare type of non-Hodgkin's lymphoma, The genetic changes associated with pathogenesis have not been well defined, This study investigates the nonrandom genetic alteration of nasal-type NWT-cell lymphoma, Methods: Nine cases were studied, Comparative genomic hybridization (CGH) was carried out using fresh tumor tissues of seven nasal-type NWT-cell lymphomas, To complement the data by CGH, loss of heterozygosity (LOH) of chromosomes 6q, 1p, and 17p using polymorphic markers and p53 gene mutation by polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) were analyzed. Results: The DNA copy number changes of seven nasal-type NWT-cell lymphomas were gains on chromosomes 2q(5), 13q(4), 10q(3), 21q(2), 3q(2), 5q(2), and 17q(2), and losses involving chromosomes 1p(4), 17p(4), 12q(3), 13q(2), and 6q(1), One of six cases informative for at least two markers for chromosome 6q showed LOH at D6S300, D6S1639, D6S261, D6S407, and D6S292, Two cases showing loss of 1p and 17q by CGH revealed LOH at D1S214, D1S503, and D17S559. P53 mutation was detected in exon 8 in one of nine cases, Conclusion: Frequent DNA losses at 1p, 17p, and 12q and gains at 2q, 13q, and 10q suggested that these regions could be targets for further molecular genetic analysis to investigate tumor suppressor genes or oncogenes associated with tumorigenesis of NWT-cell lymphoma. Infrequent alteration of 6q contrary to previous studies raises doubt about an implication of 6q loss in the pathogenesis of early-stage NWT-cell lymphoma, Further studies on more defined cases are required to verify their association. (C) 2001 Wiley-Liss, Inc.