Alteration of neural tissue structure by expression of polysialic acid induced by viral delivery of PST polysialyltransferase.
Alteration of neural tissue structure by expression of polysialic acid induced by viral delivery of PST polysialyltransferase.
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PST 聚唾液酸转移酶的病毒递送诱导聚唾液酸表达改变神经组织结构。
DOI：
10.1093/glycob/cwh014
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Rutishauser,Urs
中科院分区：
文献类型：
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作者：
Canger,AnthonyK;Rutishauser,Urs
已知神经细胞粘附分子（NCAM）上的聚唾液酸（PSA）的表达减弱细胞-细胞相互作用。在神经发育过程中，PSA-NCAM的广泛表达为神经元和神经胶质前体的迁移以及轴突的引导和靶向创造了允许的条件。NCAM多聚唾液酸化可以通过两种特异性唾液酸转移酶ST 8 SiaII（STX）和ST 8 SiaIV（PST）中的任一种发生，本研究的目的是确定逆转录病毒递送PST或STX是否可以诱导体内PSA表达，从而改变组织可塑性。将表达GFP-PST或GFP-STX的逆转录病毒注射到胚胎视网膜中，通过检查神经上皮结构、特定细胞类型的标记物的表达、细胞增殖和凋亡来评估发育。选择小鸡视网膜是因为它在发育早期下调PSA，并且具有高度定型的形态发生程序。PST的逆转录病毒表达诱导PSA在视网膜中的表达，并导致严重的，但局部的视网膜形态发生的改变，包括放射状胶质细胞形态的早期破坏，高度紊乱的视网膜层，和色素细胞侵入神经视网膜。相反，STX的逆转录病毒递送不诱导PSA表达或影响形态发生。这些发现表明，PSA的表达足以促进相对非可塑性神经组织的形态学改变。
The expression of polysialic acid (PSA) on neural cell adhesion molecule (NCAM) is known to attenuate cell–cell interactions. During neural development the widespread expression of PSA-NCAM creates permissive conditions for the migration of neuronal and glial precursors and the guidance and targeting of axons. NCAM polysialylation can occur via either of two specific sialyltransferases, ST8SiaII (STX) and ST8SiaIV (PST), and the purpose of this study was to determine if retroviral delivery of either PST or STX could induce PSA expressionin vivoand thereby alter tissue plasticity. Retroviruses expressing GFP-PST or GFP-STX were injected into embryonic retina, and development was evaluated by examining neuroepithelial structure, the expression of markers for specific cell types, cellular proliferation, and apoptosis. Chick retina was chosen because it down-regulates PSA early in its development and has a highly stereotyped program of morphogenesis. Retroviral expression of PST induced PSA expression in retina and resulted in severe but localized alterations in retinal morphogenesis, including an early disruption of radial glial cell morphology, highly disorganized retinal layers, and invasion of pigmented cells into the neural retina. In contrast, retroviral delivery of STX did not induce PSA expression or affect morphogenesis. These findings demonstrate that expression of PSA is sufficient to promote morphological alterations in a relatively nonplastic neural tissue.