Artesunate suppresses the viability and mobility of prostate cancer cells through UCA1, the sponge of miR-184.
Artesunate suppresses the viability and mobility of prostate cancer cells through UCA1, the sponge of miR-184.
复制标题
青蒿琥酯通过 UCA1（miR-184 的海绵）抑制前列腺癌细胞的活力和迁移性
DOI：
10.18632/oncotarget.15353
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发表时间：
2017-03-14
期刊：
影响因子：
--
通讯作者：
Hu H
中科院分区：
文献类型：
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作者：
Zhou Y;Wang X;Zhang J;He A;Wang YL;Han K;Su Y;Yin J;Lv X;Hu H
青蒿琥酯(ART)是一种从中国草本植物青蒿叶中分离出来的倍半萜内酯。在这里，我们评估了ART对前列腺癌(Pca)细胞株DU145和LNCaP的作用，并探讨了其可能的机制。ART抑制DU145和LNCaP细胞的活力和运动能力。从机制上讲，我们发现UCA1是泌尿系统恶性肿瘤中最重要的lncRNAs之一，可能是ART抑制肿瘤功能的一个潜在的介体。首先，暴露于ART后，UCA1水平显著降低。此外，与增生性前列腺癌组织相比，前列腺癌组织中UCA1表达上调，且UCA1水平较高预示着PCa患者预后不良。此外，将UCA1重新导入PCa细胞，逆转了ART对细胞凋亡和转移能力的影响。在此基础上，我们确定miR-184/Bcl-2轴可能是ART治疗后UCA1的下游信号通路。UCA1通过其种子序列与miR-184结合，可作为miR-184的海绵。
Artesunate (ART) is a sesquiterpene lactone isolated from the leafy portions of the Chinese herb Artemisia annua. Here, we evaluated the effect of ART on the prostate cancer (PCa) cell lines DU145 and LNCaP and explored its potential mechanisms. ART inhibited the viability and mobility of DU145 and LNCaP cells. Mechanistically, we found that UCA1, one of the most important lncRNAs in malignancies of the urinary system, may be a potential mediator contributing to the tumor suppressor function of ART. First, the UCA1 level was reduced significantly after being exposed to ART. In addition, UCA1 was up-regulated in prostate cancer tissues compared to hyperplastic prostatic tissues, and a higher UCA1 level predicted poor prognosis in PCa patients. Furthermore, reintroduction of UCA1 into PCa cells reversed the effect of ART on apoptosis and metastatic ability. Then we determined that the miR-184/Bcl-2 axis might be the downstream signaling pathway of UCA1 upon ART treatment. UCA1 binds to miR-184 through its seed sequences and may function as a sponge for miR-184.