IMPAIRMENT OF T-CELL-DEPENDENT B-CELL RESPONSES AND B-1 CELL-DEVELOPMENT IN CD19-DEFICIENT MICE
IMPAIRMENT OF T-CELL-DEPENDENT B-CELL RESPONSES AND B-1 CELL-DEVELOPMENT IN CD19-DEFICIENT MICE
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DOI：
10.1038/376352a0
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发表时间：
1995-07-27
期刊：
影响因子：
64.8
通讯作者：
ROES, J
中科院分区：
文献类型：
--
作者：
RICKERT, RC;RAJEWSKY, K;ROES, J
CD19是B谱系的标志性分化抗原。它的早期表达暗示了在B细胞发育的抗原独立阶段中CD19的作用，而在成熟的B细胞中，CD19可以与表面免疫球蛋白协同作用以诱导激活（1）。我们已经产生了CD19缺陷型小鼠，发现常规B细胞的发展不受干扰。然而，成熟的CD19（ - / - ）B细胞在响应需要T细胞帮助的蛋白质抗原方面表现出严重的缺乏。这伴随着缺乏生发中心的形成和血清抗体的亲和力成熟。因此，CD19对于通过T细胞依赖性抗原的初始B细胞激活以及将活化细胞的成熟和/或在存储区室中的成熟和/或选择至关重要。配体驱动选择的损害也可能导致观察B-1（以前是LY-1）B细胞子集的显着降低，该子群被认为是在自我抗原和细菌抗原的控制下发展的（在综述中审查了参考。
CD19 is the hallmark differentiation antigen of the B lineage. Its early expression has implicated a role for CD19 during the antigen-independent phases of B-cell development, whereas in mature B cells CD19 can act synergistically with surface immunoglobulin to induce activation(1). We have generated CD19-deficient mice and found that development of conventional B cells is unperturbed. However, mature CD19(-/-) B cells show a profound deficiency in responding to protein antigens that require T-cell help. This is accompanied by a lack of germinal centre formation and affinity maturation of serum antibodies. Thus CD19 is crucial for both initial B-cell activation by T-cell-dependent antigens and the maturation and/or selection of the activated cells into the memory compartment. An impairment in ligand-driven selection may also be responsible for the observation of a striking reduction in the B-1 (formerly Ly-1) B-cell subset, thought to develop under the control of self-antigens and bacterial antigens (reviewed in ref. 2).