Short Report: Genetic Diversity of Thottapalayam Virus, a Hantavirus Harbored by the Asian House Shrew (Suncus murinus) in Nepal
Short Report: Genetic Diversity of Thottapalayam Virus, a Hantavirus Harbored by the Asian House Shrew (Suncus murinus) in Nepal
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DOI：
10.4269/ajtmh.2011.11-0034
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发表时间：
2011-09-01
影响因子：
3.3
通讯作者：
Yanagihara, Richard
中科院分区：
文献类型：
--
作者：
Kang, Hae Ji;Kosoy, Michael Y.;Yanagihara, Richard
尽管最近在相距较远的地理区域的多个物种的鼩鼱中发现了具有遗传差异的汉坦病毒，但关于托塔帕拉亚姆病毒（TPMV）的遗传多样性和系统发育地理学的数据尚无，该病毒最初是从四十多年前在印度南部捕获的亚洲家鼩（Suncus murinus）中分离出来的。为了弥补这一知识差距，通过逆转录聚合酶链式反应，从 1996 年 1 月至 9 月在尼泊尔捕获的亚洲家鼩的档案肺组织中扩增了汉坦病毒 RNA 的 S、M 和 L 片段。配对比对和比较揭示了与原型 TPMV 大约 80% 的核苷酸序列和 > 94% 的氨基酸序列相似性。通过最大似然法和贝叶斯方法进行的系统发育分析显示，TPMV 具有地理特异性聚类，与啮齿动物和母猴传播的汉坦病毒观察到的相似。这些发现证实亚洲家鼩是 TPMV 的天然宿主，并表明存在长期的病毒宿主关系。
Despite the recent discovery of genetically divergent hantaviruses in shrews of multiple species in widely separated geographic regions, data are unavailable about the genetic diversity and phylogeography of Thottapalayam virus (TPMV), a hantavirus originally isolated from an Asian house shrew (Suncus murinus) captured in southern India more than four decades ago. To bridge this knowledge gap, the S, M, and L segments of hantavirus RNA were amplified by reverse transcription polymerase chain reaction from archival lung tissues of Asian house shrews captured in Nepal from January to September 1996. Pair-wise alignment and comparison revealed approximately 80% nucleotide and > 94% amino acid sequence similarity to prototype TPMV. Phylogenetic analyses, generated by maximum likelihood and Bayesian methods, showed geographic-specific clustering of TPMV, similar to that observed for rodent- and soricid-borne hantaviruses. These findings confirm that the Asian house shrew is the natural reservoir of TPMV and suggest a long-standing virus host relationship.