Use of qPCR and Genomic Sequencing to Diagnose Plasmodium ovale wallikeri Malaria in a Returned Soldier in the Setting of a Negative Rapid Diagnostic Assay
Use of qPCR and Genomic Sequencing to Diagnose Plasmodium ovale wallikeri Malaria in a Returned Soldier in the Setting of a Negative Rapid Diagnostic Assay
复制标题
DOI：
10.4269/ajtmh.12-0724
复制
发表时间：
2013-09-01
影响因子：
3.3
通讯作者：
Zapor, Michael
中科院分区：
文献类型：
--
作者：
Cohen, Robert;Feghali, Karla;Zapor, Michael
卵形疟原虫是已知引起人类疾病的几种临床相关疟疾物种之一。然而，与引起大多数人类疟疾病例的恶性疟原虫和间日疟原虫相反，胚珠疟原虫在热带地区分布广泛，但流行率较低。在这里，我们报告的情况下，一名士兵从利比里亚返回与卵形疟原虫疟疾。该病例突出了显微镜和疟疾快速诊断测试在诊断卵形疟原虫感染和区分卵形疟原虫与卵形疟原虫的局限性。据我们所知，这是第一个病例报告，其中定量实时聚合酶链反应扩增使用细胞色素B基因，再加上基因组测序的potra位点，用于明确诊断卵形疟原虫wallikeri疟疾。
Plasmodium ovale is one of several clinically relevant malaria species known to cause disease in humans. However, in contrast to Plasmodium falciparum and Plasmodium vivax, which are responsible for most cases of human malaria, P. ovule has a wide distribution but low prevalence in tropical regions. Here, we report the case of a soldier returning from Liberia with P. ovale wallikeri malaria. This case highlights the limitations of both microscopy and the malaria rapid diagnostic test for diagnosing infection with P. ovale and for distinguishing P. ovale wallikeri from P. ovale curtisi. To our knowledge, this is the first case report in which quantitative real-time polymerase chain reaction amplification using the Cytochrome B gene, coupled with genomic sequencing of the potra locus, was used for definitive diagnosis of P. ovale wallikeri malaria.