Glycerol production by a novel osmotolerant yeast Candida glycerinogenes
Glycerol production by a novel osmotolerant yeast Candida glycerinogenes
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DOI：
10.1007/s002530100596
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发表时间：
2001-06
影响因子：
5
通讯作者：
J. Zhuge;H. Fang;Zhengliang Wang;D. Chen;Hyuck Jin;H.-L. Gu
中科院分区：
文献类型：
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作者：
J. Zhuge;H. Fang;Zhengliang Wang;D. Chen;Hyuck Jin;H.-L. Gu
产甘油假丝酵母是一种在高渗透压环境下从天然样品中分离出来的耐渗透酵母菌，具有适度的糖耐受性和极高的甘油产量。研究了丙三醇生成的最佳条件。甘油原的温度为29-33℃，pH为4-6。产甘油的最佳培养基为葡萄糖230 ~ 250 g /l、尿素2 g /l、玉米浆5 ml /l（磷酸盐55 ~ 65 mg /l）；pH值未调整。以消耗葡萄糖240 g/l为基准，甘油的最高产率为64.5% (w/w)，甘油的最高浓度为137 g/l。这些结果是在最佳发酵条件下使用30-l的搅拌发酵罐得到的。在5万l气升发酵罐中进行10次分批发酵，平均葡萄糖240.6 g/l，平均甘油产量为50.67% (w/w)，平均甘油浓度为121.9 g/l。
Candida glycerinogenes,an osmotolerant yeast isolated from a natural sample in an environment of high osmotic pressure, had a modest sugar-tolerance and an extremely high glycerol productivity. The optimum conditions for glycerol formation byC. glycerinogeneswere a temperature of 29–33 °C and a pH of 4–6. The optimum medium for glycerol production consisted of 230–250 g glucose/l, 2 g urea/l and 5 ml corn steep liquor/l (55–65 mg phosphates/l); the pH was not adjusted. The highest yield of glycerol was 64.5% (w/w) based on consumed glucose from 240 g glucose/l, and the highest concentration of glycerol was 137 g/l from 260 g glucose/l. These results were obtained by using a 30-l agitated fermentor under optimal fermentation conditions. In ten batch-fermentations carried out in a 50,000-l airlift fermentor, an average yield of glycerol of 50.67% (w/w) and an average glycerol concentration of 121.9 g/l were obtained from an average 240.6 g glucose/l.