A comparative dose-response study of cartilage-derived morphogenetic protein (CDMP)-1,-2 and-3 for tendon healing in rats
A comparative dose-response study of cartilage-derived morphogenetic protein (CDMP)-1,-2 and-3 for tendon healing in rats
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DOI：
10.1016/s0736-0266(03)00010-x
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发表时间：
2003-07-01
影响因子：
2.8
通讯作者：
Aspenberg, P
中科院分区：
文献类型：
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作者：
Forslund, C;Rueger, D;Aspenberg, P
软骨来源的形态发生蛋白（CDMPs）属于骨形态发生蛋白（BMP）家族，已知是软骨和骨诱导剂，以及诱导肌腱和韧带样组织。在这项研究中，我们研究了四种不同剂量（0,0.4,2和10马克杯）的CDMP-1， -2或-3对大鼠模型肌腱愈合的影响，以及不同cdmp和剂量之间成骨的差异。在110只大鼠中，通过2毫米的皮肤切口切除3毫米的跟腱段。术后6 h向缺损处注射CDMP-1、-2或-3。术后8 d处死大鼠。对肌腱再生进行生物力学测试。所有三种CDMPs的强度和刚度都有显著的剂量相关增加，但CDMPs之间没有发现差异。另用50只大鼠比较最高剂量的CDMPs与对照组和成骨蛋白1 （OP-1）在4周后的软骨或骨形成情况。软骨出现在所有组，包括对照组。除对照组外，所有组中的一些标本都含有骨。CDMPs之间没有差异。CDMP-1、CDMP-3和OP-1组的钙含量明显高于对照组。只有CDMP-2组和对照组的钙含量明显低于OP-1组。总之，在这种情况下，三种CDMPs在改善肌腱修复和成骨性方面表现相似。(C) 2003骨科研究学会。Elsevier Science Ltd.出版。版权所有。
Cartilage-derived morphogenetic proteins (CDMPs), belonging to the bone morphogenetic protein (BMP) family, are known to be cartilage and bone inducers as well as to induce tendon and ligament-like tissue. In this study we investigated the influence of CDMP-1, -2 or -3 at four different doses (0, 0.4, 2 and 10 mug) on tendon healing in a rat model, as well as differences in osteogenesis between the different CDMPs and doses.In 110 rats, a 3 mm segment of the Achilles tendon was removed via a 2 mm skin incision. CDMP-1, -2 or -3 was injected into the defect 6 h postoperative. The rats were killed 8 days after operation. The tendon regenerates were tested biomechanically. There was a significant dose-related increase in strength and stiffness with all three CDMPs, but no difference between the CDMPs was found.Another 50 rats were used to compare the highest dose of the CDMPs with controls and osteogenic protein 1 (OP-1), as regards cartilage or bone formation after 4 weeks. Cartilage occurred in all groups, including the controls. Some specimens in all groups contained bone, except the controls. No difference between the CDMPs could be demonstrated. The CDMP-1, CDMP-3 and OP-1 groups contained significantly more calcium than controls. Only the CDMP-2 group and the controls contained significantly less calcium than the OP-1 group.In conclusion, the three CDMPs appeared similar as regards improvement of tendon repair and osteogenicity in this setting. (C) 2003 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.