Ectomycorrhizas involving sebacinoid mycobionts.
Ectomycorrhizas involving sebacinoid mycobionts.
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涉及癸二酸类真菌生物的外生菌根。
DOI：
10.1017/s0953756202007116
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发表时间：
2003
影响因子：
--
通讯作者：
R. Bauer
中科院分区：
文献类型：
--
作者：
A. Urban;M. Weiß;R. Bauer
采用直接扩增法，从野外采集的外生菌根样品中检测了Sebacinaceae的核核糖体DNA特征序列。本研究证实了由Sebacinaceae形成的外生菌根，并从遗传学、形态学和超微结构上对代表性样品进行了鉴定。调查sebacinoid菌根有足够的特征，以确定形态。它们的特点是一个特征，clampless，亲水extramatical菌丝体，这是非常可变的直径和壁厚，并通过存在独特的，Y形，膨胀的多分支。地幔结构的差异清楚地区分两个调查sebacinoid菌根类型。核大亚基假基因的D1/D2结构域和ITS 1和ITS 2区域的比较确定了真菌的合作伙伴之一的菌根样品作为Sebacina incrustans。外生菌根的超微结构调查显示一致的Sebacinaceae的doliporus/parenthesome架构。将最近发表的从Sebacinoid菌根获得的序列数据与我们的序列进行比较，并讨论了Sebacinaceae中复杂的营养关系。对外生菌根和担子的观察表明，在各种外生菌根植物群落中，Sebacinaceae是相当常见的真菌。
Nuclear ribosomal DNA sequences characteristic of Sebacinaceae were detected by direct amplification of DNA from field collected ectomycorrhizal samples. A study was undertaken to confirm the formation of ectomycorrhizas by Sebacinaceae and to characterise representative samples genetically, morphologically and ultrastructurally. The investigated sebacinoid mycorrhizas were sufficiently characteristic to be identified morphologically. They are distinguished by a characteristic, clampless, hydrophilic extramatrical mycelium, which is very variable in diameter and in wall thickness, and by the presence of distinctive, y-shaped, inflated multibranchings. Differences in the mantle structure clearly discriminate the two investigated sebacinoid mycorrhizal types. Comparison of the D1/D2 domains of the nuclear large subunit pseudogene and the ITS1 and ITS2 regions identifies the fungal partner of one of the mycorrhizal samples as Sebacina incrustans. Ultrastructural investigations of the ectomycorrhizas show a doliporus/parenthesome architecture consistent with that of the Sebacinaceae. Recently published sequence data obtained from sebacinoid mycorrhizas are compared to our sequences and the complex trophic relationships in the Sebacinaceae are discussed. Observations on ectomycorrhizas and basidiomes suggest that species of Sebacinaceae are fairly common mycobionts in various ectomycorrhizal plant communities.