Consequences of Photoselection on the Intensity and Polarization of Luminescent Molecules
Consequences of Photoselection on the Intensity and Polarization of Luminescent Molecules
复制标题
光选择对发光分子的强度和偏振的影响
DOI：
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发表时间：
1968
期刊：
影响因子：
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通讯作者：
A. Kalantar
中科院分区：
文献类型：
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作者：
A. Kalantar
如果旋转弛豫时间与激发态寿命相比慢，则光学激发的发光分子通常不是随机取向的，即，分子被光选择。发射的属性进行检查，并适用于相对强度测量。发射强度的分析表明，相对强度测量（例如，荧光与磷光量子产率的比率）在吸收和发射轴的关系改变时是错误的。对发射光偏振态的分析表明了如何识别和避免误差，以及如何通过适当选择以下变量来提高相对强度测定的灵敏度：激发光的偏振态、激发光和发射光之间的角度以及发射光的偏振态。
Optically excited luminescent molecules are often not randomly oriented if rotational relaxation times are slow compared to excited‐state lifetimes, i.e., the molecules are photoselected. The properties of the emission are examined and applied to relative intensity measurements. Analysis of the emission intensity shows that relative intensity measurements (e.g., the ratio of fluorescence to phosphorescence quantum yields) are in error whenever the relation of the axes of absorption and emission changes. Analysis of the polarization of the emission shows how to recognize and avoid the errors and how to enhance the sensitivity of relative intensity determinations by suitable selection of the following variables: polarization of the exciting light, angle between the exciting and the emitted beams, and the polarization of the emitted light.