Functional analysis of a fatty acid elongase from arachidonic acid-producing Mortierella alpina 1S-4
Functional analysis of a fatty acid elongase from arachidonic acid-producing Mortierella alpina 1S-4
复制标题
DOI：
10.1007/s00253-008-1675-z
复制
发表时间：
2008-09
影响因子：
5
通讯作者：
E. Sakuradani;S. Murata;H. Kanamaru;S. Shimizu
中科院分区：
文献类型：
--
作者：
E. Sakuradani;S. Murata;H. Kanamaru;S. Shimizu
我们描述了一个基因（MAELO）的分离和表征，该基因编码的脂肪酸延伸酶从花生四烯酸生产真菌被孢霉alpina 1 S-4。尽管同源MAELO基因已经从mM中分离出来。alpinaATCC 32221，其功能尚未确定。来自mM. alpina 1 S-4在酿酒酵母中的表达证实其编码脂肪酸延伸酶。酵母菌的脂肪酸组成的分析揭示了22-，24-和26-碳饱和脂肪酸的积累。另一方面，对M. alpina 1 S-4进行。与野生菌株相比，通过RNA干扰获得的基因沉默菌株表现出低含量的20-，22-和24-碳饱和脂肪酸和高含量的硬脂酸（18个碳）。由该MAb基因编码的酶被证明参与M中20-、22-和24-碳饱和脂肪酸的生物合成。阿尔卑斯山1 S-4。
We describe the isolation and characterization of a gene (MAELO) that encodes a fatty acid elongase from arachidonic acid-producing fungusMortierella alpina1S-4. Although the homologousMAELOgene had already been isolated fromM. alpinaATCC 32221, its function had not yet been identified. TheMAELOgene fromM. alpina1S-4 was confirmed to encode a fatty acid elongase by its expression in yeastSaccharomyces cerevisiae. Analysis of the fatty acid composition of the yeast transformant revealed the accumulation of 22-, 24-, and 26-carbon saturated fatty acids. On the other hand, RNA interference of theMAELOgene inM. alpina1S-4 was carried out. The gene-silenced strain obtained on RNA interference exhibited low contents of 20-, 22-, and 24-carbon saturated fatty acids and a high content of stearic acid (18 carbons), compared with those in the wild strain. The enzyme encoded by theMAELOgene was demonstrated to be involved in the biosynthesis of 20-, 22-, and 24-carbon saturated fatty acids inM. alpina1S-4.