THE LIFE-SPAN OF OSTEOCLASTS - EXPERIMENTAL STUDIES USING THE GIANT GRANULE CYTOPLASMIC MARKER CHARACTERISTIC OF BEIGE MICE
THE LIFE-SPAN OF OSTEOCLASTS - EXPERIMENTAL STUDIES USING THE GIANT GRANULE CYTOPLASMIC MARKER CHARACTERISTIC OF BEIGE MICE
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DOI：
10.1016/8756-3282(85)90223-6
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发表时间：
1985-01-01
期刊：
影响因子：
4.1
通讯作者：
SEIFERT, MF
中科院分区：
文献类型：
--
作者：
MARKS, SC;SEIFERT, MF
破骨细胞是大的多核骨骼细胞，由血源性单核前体细胞融合而成。与单核前体细胞的融合发生在整个生命过程中，破骨细胞的生存被认为依赖于融合后的持续补充。这项研究检测了正常小鼠的破骨细胞寿命，定义为不融合的最大存活时间，这些小鼠接受了消除宿主干细胞的照射，并用来自米色(BG)小鼠的干细胞拯救了破骨细胞具有独特表型的小鼠。供体表型的破骨细胞在第二周出现，并逐渐增多，到第六周，宿主表型的破骨细胞消失。单纯放射治疗不会引起破骨细胞表型的任何改变。这些数据被解释为表明，未融合前体的破骨细胞最大存活时间不到6周。
Osteoclasts are large multinucleated skeletal cells that form by fusion of bloodborne mononuclear precursors. Fusion with mononuclear precursors occurs throughout life, and survival of osteoclasts is believed to be dependent upon continued replenishment by fusion. This study examined osteoclast lifespan, defined as maximal survival without fusion, in normal mice irradiated to eliminate host stem cells and rescued with stem cells from beige (bg) mice whose osteoclasts have a distinctive phenotype. Osteoclasts of donor phenotype appeared during the second week and progressively increased so that by the sixth week no osteoclasts of host phenotype were present. Radiation alone did not produce any change in osteoclast phenotype. These data are interpreted to indicate that the maximal survival of osteoclasts without fusion of precursors is less than 6 weeks.