Eppin: An effective target for male contraception
Eppin: An effective target for male contraception
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DOI：
10.1016/j.mce.2005.12.039
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发表时间：
2006-05-16
影响因子：
4.1
通讯作者：
Richardson, R. T.
中科院分区：
文献类型：
--
作者：
O'Rand, M. G.;Widgren, E. E.;Richardson, R. T.
Eppin（附睾蛋白酶抑制剂）是乳清酸性蛋白（WAP）型四二硫键核心（WFDC）基因家族的成员。这项研究提供了有关人类 20 号染色体 Eppin 簇内 Eppin 和 Eppin 样基因的最新信息。Eppin 蛋白的虚拟结构模型表明 Eppin 的 C 端一半在结构上与 Kunitz 型胰蛋白酶抑制剂同源。 Eppin N 末端可能与防御素型分子具有结构相似性，而不是与 WAP 共有序列相似。人类精子有 Eppin 受体。当重组精麦蛋白 (Sg) 用 PSA 消化时，会产生许多低分子量片段。然而，当 Eppin 与 Sg 结合时，PSA 的消化就会受到调节。在 Eppin 的 C 末端添加抗体可阻断 PSA 活性调节。我们可以根据对 Eppin 上的抗 Eppin 表位的分析推测，当抗 Eppin 抗体与精子表面的 Eppin 结合时，它们会阻断精液蛋白的结合位点。 (c) 2005 Elsevier Ireland Ltd. 保留所有权利。
Eppin (epididymal protease inhibitor) is a member of the whey acidic protein (WAP)-type four-disulfide core (WFDC) gene family. This study provides updated information on Eppin and the Eppin-like genes within the Eppin cluster on human chromosome 20. A virtual structural model of the Eppin protein demonstrates that the C-terminal half of Eppin is structurally homologous to the Kunitz-type trypsin inhibitor. The Eppin N-terminal may have structural similarities to defensin-type molecules, rather than to that of the WAP consensus sequence. Human spermatozoa have a receptor for Eppin. When recombinant semenogelin (Sg) is digested with PSA many low molecular weight fragments are produced. However, when Eppin is bound to Sg, digestion by PSA is modulated. Addition of antibodies to the C-terminal of Eppin resulted in blocking PSA activity modulation. We can hypothesize from our analysis of anti-Eppin epitopes on Eppin that when anti-Eppin antibodies are bound to Eppin on the sperm surface they block the binding site for semenogelin. (c) 2005 Elsevier Ireland Ltd. All rights reserved.