Fabrication and characterization of PCL/gelatin composite nanofibrous scaffold for tissue engineering applications by electrospinning method
Fabrication and characterization of PCL/gelatin composite nanofibrous scaffold for tissue engineering applications by electrospinning method
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DOI：
10.1016/j.msec.2012.12.015
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发表时间：
2013-04-01
影响因子：
7.9
通讯作者：
Mishra, Narayan Chandra
中科院分区：
文献类型：
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作者：
Gautam, Sneh;Dinda, Amit Kumar;Mishra, Narayan Chandra
在本研究中，复合纳米纤维组织工程支架组成的聚己内酯和明胶，通过静电纺丝方法，使用一种新的成本效益的溶剂混合物：氯仿/甲醇的聚己内酯（PCL）和乙酸的明胶。纳米纤维支架的形态进行了研究，通过使用场发射扫描电子显微镜（FE-SEM），这清楚地表明，纳米纤维的形态是由溶液中的PCL明胶的重量比的影响。只有当PCL/明胶的重量比足够高（10：1）时才产生均匀的纤维。通过傅里叶变换红外光谱（FT-IR）、热重分析（TG）和X射线衍射（XRD）对支架进行了表征。FT-IR和TG分析表明支架内PCL和明胶分子之间存在一定的相互作用，而XRD结果表明PCL/明胶复合支架具有结晶性。通过MIT试验评价支架对L929小鼠成纤维细胞的细胞毒性作用，并通过DNA定量证实支架上的细胞增殖。MU测定和DNA定量L929小鼠成纤维细胞的阳性结果表明，由天然聚合物（明胶）和合成聚合物（PCL）的组合制成的支架可以作为组织工程应用的良好候选物。(C)2012 Elsevier B. V.保留所有权利。
In the present study, composite nanofibrous tissue engineering-scaffold consisting of polycaprolactone and gelatin, was fabricated by electrospinning method, using a new cost-effective solvent mixture: chloroform/methanol for polycaprolactone (PCL) and acetic acid for gelatin. The morphology of the nanofibrous scaffold was investigated by using field emission scanning electron microscopy (FE-SEM) which clearly indicates that the morphology of nanofibers was influenced by the weight ratio of PCL to gelatin in the solution. Uniform fibers were produced only when the weight ratio of PCL/gelatin is sufficiently high (10:1). The scaffold was further characterized by Fourier transform infrared (FT-IR) spectroscopy, thermogravimetric (TG) analysis, and X-ray diffraction (XRD). FT-IR and TG analysis indicated some interactions between PCL and gelatin molecules within the scaffold, while XRD results demonstrated crystalline nature of PCL/gelatin composite scaffold. Cytotoxicity effect of scaffold on L929 mouse fibroblast cells was evaluated by MIT assay and cell proliferation on the scaffold was confirmed by DNA quantification. Positive results of MU assay and DNA quantification L929 mouse fibroblast cells indicated that the scaffold made from the combination of natural polymer (gelatin) and synthetic polymer (PCL) may serve as a good candidate for tissue engineering applications. (C) 2012 Elsevier B.V. All rights reserved.