COMBINED STAINING METHOD FOR THE DEMONSTRATION OF TISSUE EOSINOPHILS AND MAST-CELLS
COMBINED STAINING METHOD FOR THE DEMONSTRATION OF TISSUE EOSINOPHILS AND MAST-CELLS
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DOI：
10.1179/his.1993.16.2.143
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发表时间：
1993-06-01
影响因子：
1.1
通讯作者：
MATTHEWS, HR
中科院分区：
文献类型：
--
作者：
DUFFY, JP;SMITH, PJ;MATTHEWS, HR
肥大细胞和嗜酸性粒细胞的定量在变应性或寄生虫性组织炎症的研究中是有价值的。我们描述了一种新的方法，结合两个可靠的染料，阿斯特拉蓝和活力新红，染色肥大细胞和嗜酸性粒细胞在同一组织切片。肥大细胞呈紫蓝色，嗜酸性粒细胞呈鲜红色。因为该方法对已经用甲醛盐水或缓冲甲醛固定的组织中的两种细胞进行染色，所以它可以用于已经经历标准固定的存档组织。背景的最小染色使得对两种细胞的定量研究变得简单，即使在低放大倍数下也是如此。
Quantitation of mast cells and eosinophils is of value in studies of allergic or parasitic tissue inflammation. We describe a new method that combines two reliable stains, astra blue and vital new red, to stain both mast cells and eosinophils in the same tissue section. The mast cells stain violet-blue and the eosinophils bright red. Because this method stains both cells in tissue that has been fixed with formol saline or buffered formaldehyde, it can be used on archival tissue that has undergone standard fixation. The minimal staining of the background makes quantitative studies of both cells simple, even at low magnification.