Assembly of the Synaptonemal Complex Is a Highly Temperature-Sensitive Process That Is Supported by PGL-1 During Caenorhabditis elegans Meiosis.
Assembly of the Synaptonemal Complex Is a Highly Temperature-Sensitive Process That Is Supported by PGL-1 During Caenorhabditis elegans Meiosis.
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DOI：
10.1534/g3.112.005165
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发表时间：
2013-04-09
期刊：
影响因子：
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通讯作者：
Nabeshima K
中科院分区：
文献类型：
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作者：
Bilgir C;Dombecki CR;Chen PF;Villeneuve AM;Nabeshima K
在减数分裂期间成功的染色体分离取决于联会复合体（SC），一种稳定对齐的同源染色体之间配对的结构。在这里，我们表明，SC大会是一个温度敏感的过程中秀丽隐杆线虫减数分裂。SC组装的温度敏感性最初是通过鉴定生殖系特异性P-颗粒组分PGL-1作为促进稳定同源配对的因子来揭示的。使用监测体内同源物配对的测定系统，我们表明在25°下PGL-1的耗尽破坏同源物配对。对pgl-1−null突变体中其他染色体位点的同源配对进行分析，发现其配对缺陷与缺乏SC中心区成分的突变体中观察到的配对缺陷相似。此外，温度≥25°时pgl-1功能的丧失导致SC中心区组分SYP-1加载到染色体上的严重受损，导致SYP-1聚集体的形成。SC组装在野生型蠕虫中也是温度敏感的，其在≥26.5° C的温度下表现出类似的SYP-1加载缺陷和SYP-1聚集体的形成。温度变化分析表明，SC的组装对温度敏感，但SC的维护不敏感。我们认为，SC组装的温度敏感性（ts）的性质可能有助于健身和适应能力，在C。通过使减数分裂破坏响应环境变化，从而增加生产的雄性后代可用于异交。
Successful chromosome segregation during meiosis depends on the synaptonemal complex (SC), a structure that stabilizes pairing between aligned homologous chromosomes. Here we show that SC assembly is a temperature-sensitive process during Caenorhabditis elegans meiosis. Temperature sensitivity of SC assembly initially was revealed through identification of the germline-specific P-granule component PGL-1 as a factor promoting stable homolog pairing. Using an assay system that monitors homolog pairing in vivo, we showed that depletion of PGL-1 at 25° disrupts homolog pairing. Analysis of homolog pairing at other chromosomal loci in a pgl-1−null mutant revealed a pairing defect similar to that observed in mutants lacking SC central region components. Furthermore, loss of pgl-1 function at temperatures ≥25° results in severe impairment in loading of SC central region component SYP-1 onto chromosomes, resulting in formation of SYP-1 aggregates. SC assembly is also temperature sensitive in wild-type worms, which exhibit similar SYP-1 loading defects and formation of SYP-1 aggregates at temperatures ≥26.5°. Temperature shift analyses suggest that assembly of the SC is temperature sensitive, but maintenance of the SC is not. We suggest that the temperature sensitive (ts) nature of SC assembly may contribute to fitness and adaptation capacity in C. elegans by enabling meiotic disruption in response to environmental change, thereby increasing the production of male progeny available for outcrossing.