Chemically induced murine erythroleukemic differentiation.
Chemically induced murine erythroleukemic differentiation.
复制标题
化学诱导小鼠红白血病分化。
DOI：
10.1016/0304-419x(80)90015-3
复制
发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Marks,PA
中科院分区：
文献类型：
--
作者：
Reuben,RC;Rifkind,RA;Marks,PA
Friend病毒转化的小鼠红白血病细胞（MEL细胞）系[1]提供了一个模型系统，用于研究细胞生理和基因活性的改变，这些改变导致红细胞分化的形态学、生理和生化变化。（审查见参考文献2和3）。有几个特点有助于这种细胞系统的有用性：细胞系可以通过悬浮培养的连续传代无限期地维持，可以在半固体培养基中克隆；用几种化合物中的一种培养可以诱导细胞表达其红系特征；可以获得大量未诱导或诱导分化的细胞，用于生化分析；对某些诱导剂有抗性的变异已经被分离出来。MEL细胞很可能是停留在发育阶段的转化细胞，其发育阶段已经局限于红细胞生成分化[4-8]。对这一系统的发育调控机制的理解应该能够阐明在正常造血中活跃的类似控制。本实验室主要研究红体诱导分化的两个方面：一是红体发育过程中大分子编码基因的表达；其次，诱导剂对细胞增殖及与细胞周期事件相关的分化特性表达的影响。
The Friend virus-transformed murine erythroleukemia cell (MEL cell) line [1] provides a model system in which to study alterations in cellular physiology and gene activity which result in the morphological, physiological, and biochemical changes characteristic of erythroid differentiation.(For review see Refs. 2 and 3). Several characteristics contribute to the usefulness of this cell system: The cell line can be maintained indefinitely by serial passage in suspension culture and can be cloned in semi-solid media; cells can be induced to express their erythroid characteristics by culture with one of a number of chemical compounds; large quantities of cells, either uninduced or induced to differentiate, can be obtained for biochemical analyses; and variants that are resistant to selected inducers have been isolated. It is likely that MEL cells are transformed cells arrested at a developmental stage already restricted to erythropoietic differentiation [4-8]. An understanding of developmental regulatory mechanisms in this system should elucidate similar controls active in normal hematopoiesis.Our laboratory has focused on two general aspects of the problem of induced erythroid differentiation: firstly, the expression of genes which encode macromolecules characteristic of the erythroid developmental program; secondly, the effects of inducer on cell proliferation and the expression of differentiated characteristics in relation to cell cycle events.