Protocol on induction of TMJ articular disc degeneration in rats by utilization of botulinum toxin
Protocol on induction of TMJ articular disc degeneration in rats by utilization of botulinum toxin
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DOI：
10.1016/j.archoralbio.2010.03.018
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发表时间：
2010-07-01
影响因子：
3
通讯作者：
Palomari, Euanisi Teresa
中科院分区：
文献类型：
--
作者：
Guerra, Flavia Da Re;de Souza Pires, Ivan Luiz;Palomari, Euanisi Teresa
目的：利用肉毒杆菌毒素建立大鼠颞下颌关节椎间盘实验性退行性变模型，为今后纤维软骨退行性疾病的研究提供依据。方法：针对上述目的，选取雄性Lewis大鼠12只，分为CG组、对照组和DG组（6单位/kg）。形态学分析采用苏木精-伊红、甲苯胺蓝和Picrosirius染色的组织学切片；采用sds -聚丙烯酰胺凝胶电泳法进行生化分析。结果：退行性关节盘的DG表现出与文献描述一致的特殊特征：细胞数量减少，细胞方向和细胞外基质普遍紊乱，糖胺聚糖含量增加，组织胶原降解。结论：基于形态学和生化结果，我们认为所提出的退变模型对未来损伤和纤维软骨再生过程的研究是满意的。(C) 2010 Elsevier Ltd.版权所有。
Objective: To develop a model of experimental degeneration in the articular disc of the TMJ of rats through the use of botulinum toxin that can be used in future studies of degenerative diseases on fibrocartilage.Methods: Aiming at the above-mentioned objective, 12 Lewis male rats were used and divided into two groups: CG, control group and DG, group of animals to which the botulinum toxin was administered (6 units/kg). The morphological analysis was carried out utilizing histological cuts stained with hematoxyline-eosine, toluidine blue and Picrosirius; the biochemical analysis was made by SDS-polyacrylamide gel electrophoresis.Results: The DG showed peculiar characteristics regarding a degeneration joint disk, compatible with those described in literature as: reduction of cells number, general disorganization of cells direction and extracellular matrix, increase in glycosaminoglycans content and degradation of the tissue collagen.Conclusions: Based on the morphological and biochemical results, it was concluded that the proposed degeneration model showed to be satisfactory for futures studies of injuries and fibrocartilage regeneration processes. (C) 2010 Elsevier Ltd. All rights reserved.