AUTOFLUORESCENCE OF ADIPOSE-TISSUE MEASURED WITH FIBER OPTICS
AUTOFLUORESCENCE OF ADIPOSE-TISSUE MEASURED WITH FIBER OPTICS
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DOI：
10.1016/0309-1740(87)90074-x
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发表时间：
1987-01-01
期刊：
影响因子：
7.1
通讯作者：
SWATLAND, HJ
中科院分区：
文献类型：
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作者：
SWATLAND, HJ
用光纤直接测量猪皮下脂肪组织的局部吸光度和荧光。使用汞或氙光源通过分叉光导的一个分支照射组织样本。通过光导的另一个分支检测荧光。光栅，棱镜和连续干涉滤光单色器安装在适当的位置在光路和测量进行了计算机操作的光电倍增管，其动态范围在每个波长进行了优化测量。在420 nm处有一个小的吸收峰。在365 nm激发下，荧光发射光谱的峰值在510 nm处，具有从430至450 nm的次级平台。脯氨酸粉末和纯化的生化III型胶原蛋白也是自发荧光的，峰值发射在510 nm处。用荧光显微镜观察与牛心脏瓣膜相连的脂肪细胞，以获得在同一切片上含有脂肪细胞和所有三种组织学类型的结缔组织纤维的制备物。网状纤维（脂肪细胞周围）以及胶原蛋白和弹性蛋白纤维（心脏瓣膜中）都是自发荧光的。有人建议，脂肪组织荧光的主要来源是从周围的脂肪细胞的网状纤维，与其他来源，如胶原隔膜，血管弹性蛋白，并可能，细胞质成分的微小贡献。
The topical absorbance and fluorescence of porcine subcutaneous adipose tissue were measured directly with fibre optics. Either mercury or xenon light sources were used to illuminate tissue samples through one branch of a bifurcated light guide. Fluorescence was detected through the other branch of the light guide. Grating, prism and continuous interference filter monochromators were installed at appropriate locations in the light path and measurements were made with a computer-operated photomultiplier whose dynamic range was optimized at each wavelength measured. There was a small absorbance peak at 420 nm. With excitation at 365 nm, the peak of the fluorescence emission spectrum was at 510 nm with a secondary plateau from 430 to 450 nm. Proline powder and purified biochemical Type III collagen were also autofluorescent with a peak emission at 510 nm. Adipose cells associated with bovine heart valves were exmained by fluorescence microscopy to obtain preparations containing adipose cells plus all three histological types of connective tissue fibres on the same section. Reticular fibres (around adipose cells) together with collagen and elastin fibres (in the heart valves) were all autofluorescent. It is suggested that the major source of adipose tissue fluorescence is from the reticular fibres that surround adipose cells, with minor contributions from other sources such as collagenous septa, vascular elastin and, possibly, cytoplasmic components.