Differential composition of proteomes in sow colostrum and milk from anterior and posterior mammary glands.
Differential composition of proteomes in sow colostrum and milk from anterior and posterior mammary glands.
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DOI：
10.2527/jas.2010-2972
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发表时间：
2010-08
影响因子：
3.3
通讯作者：
Weizong Wu;Xiaoqiu Wang;Guoyao Wu;Guoyao Wu;S. Kim;F. Chen;Junjun Wang
中科院分区：
文献类型：
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作者：
Weizong Wu;Xiaoqiu Wang;Guoyao Wu;Guoyao Wu;S. Kim;F. Chen;Junjun Wang
从前、中乳腺（MG）泌乳的仔猪比吮吸后MG的仔猪生长快，但其内在机制尚不清楚。本研究的目的是研究前、后MG初乳和乳汁分泌的差异蛋白质组。使用了6头健康的经产母猪，其中7对MG;第1对和第2对被定义为前MG，第6对和第7对被定义为后MG。分别于分娩后第1天和第14天采集初乳和乳汁。比较蛋白质组学分析，以确定差异表达的蛋白质在初乳和牛奶分泌的前，后MG。结果表明，初乳和牛奶中的蛋白质组成有显着变化的解剖位置的MG。与后MG相比，前MG初乳中免疫球蛋白、乳凝集素和结合珠蛋白表达上调（P < 0.05）。前MG组乳中免疫球蛋白和乳铁蛋白含量显著高于后MG组（P < 0.05）。此外，来自体细胞的蛋白质含量在后MG组高于前MG组（P < 0.05）。大多数蛋白质，其中丰度上调初乳和牛奶从前MG，有助于被动免疫，肠道发育的哺乳仔猪和上皮完整性，和MG的健康。总的来说，这些结果表明，与后MG相比，前MG在蛋白质合成中更活跃，并在初乳和乳汁中产生更多的免疫球蛋白和乳铁蛋白。
Piglets obtaining milk from anterior and middle mammary glands (MG) grow faster than those suckling posterior MG, but the underlying mechanisms are not clear. The purpose of this study was to investigate the differential proteomes of colostrum and milk secreted by anterior and posterior MG. Six healthy primiparous sows with 7 pairs of MG were used; the first and the second pairs were defined as anterior MG and the sixth and seventh pairs as posterior MG. Colostrum and milk were collected at d 1 and 14 after parturition, respectively. Comparative proteomics analysis was performed to identify the differentially expressed proteins in colostrum and milk secreted by anterior and posterior MG. Results show that protein composition in colostrum and milk varied markedly with the anatomical location of MG. Immunoglobulins, lactadherin, and haptoglobin were upregulated (P < 0.05) in colostrum from anterior MG compared with posterior MG. Concentrations of immunoglobulins and lactoferrin in milk from anterior MG were greater (P < 0.05) than milk from posterior MG. Moreover, concentration of proteins from somatic cells was greater (P < 0.05) in milk from posterior MG compared with anterior MG. Most proteins, in which abundance was upregulated in colostrum and milk from anterior MG, contribute to passive immunity, intestinal development of suckling piglets and epithelial integrity, and the health of MG. Collectively, these results indicate that in comparison with posterior MG, anterior MG are more active in protein synthesis and produce more immunoglobulins and lactoferrin in colostrum and milk.