A genome-wide transcriptional analysis using Arabidopsis thaliana Affymetrix gene chips determined plant responses to phosphate deprivation
A genome-wide transcriptional analysis using Arabidopsis thaliana Affymetrix gene chips determined plant responses to phosphate deprivation
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DOI：
10.1073/pnas.0505266102
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发表时间：
2005-08-16
影响因子：
11.1
通讯作者：
Thibaud, MC
中科院分区：
文献类型：
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作者：
Misson, J;Raghothama, KG;Thibaud, MC
磷是植物的必需元素之一，由于其在土壤中的有效性和移动性较低，往往是一种限制性养分。植物形态和生化过程的显著变化与磷(PI)缺乏有关。然而，这些对PI缺乏反应的分子基础还没有完全阐明。因此，利用拟南芥全基因组Affymetrix基因芯片(ATH1)对22,810个基因转录丰度的时空变化进行了全面的研究。分析显示，分别有612个和254个PI反应基因被协同诱导和抑制。其中一些基因的功能分类表明，它们参与了各种代谢途径、离子转运、信号转导、转录调控和其他与生长发育相关的过程。本研究详细分析了PI饥饿诱导的拟南芥全基因组与生化过程相关的基因表达变化。这些结果不仅加深了我们对与磷缺乏相关的分子过程的了解，而且有助于识别提高作物对磷的利用的关键分子决定因素。
Phosphorus, one of the essential elements for plants, is often a limiting nutrient because of its low availability and mobility in soils. Significant changes in plant morphology and biochemical processes are associated with phosphate (Pi) deficiency. However, the molecular bases of these responses to Pi deficiency are not thoroughly elucidated. Therefore, a comprehensive survey of global gene expression in response to Pi deprivation was done by using Arabidopsis thaliana whole genome Affymetrix gene chip (ATH1) to quantify the spatio-temporal variations in transcript abundance of 22,810 genes. The analysis revealed a coordinated induction and suppression of 612 and 254 Pi-responsive genes, respectively. The functional classification of some of these genes indicated their involvement in various metabolic pathways, ion transport, signal transduction, transcriptional regulation, and other processes related to growth and development. This study is a detailed analysis of Pi starvation-induced changes in gene expression of the entire genome of Arabidopsis correlated with biochemical processes. The results not only enhance our knowledge about molecular processes associated with Pi deficiency, but also facilitate the identification of key molecular determinants for improving Pi use by crop species.